2b29

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2b29.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2b29.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2b29| PDB=2b29 | SCENE= }}
{{STRUCTURE_2b29| PDB=2b29 | SCENE= }}
-
'''N-terminal domain of the RPA70 subunit of human replication protein A.'''
+
===N-terminal domain of the RPA70 subunit of human replication protein A.===
-
==Overview==
+
<!--
-
One of many protein-protein interactions modulated upon DNA damage is that of the single-stranded DNA-binding protein, replication protein A (RPA), with the p53 tumor suppressor. Here we report the crystal structure of RPA residues 1-120 (RPA70N) bound to the N-terminal transactivation domain of p53 (residues 37-57; p53N) and, by using NMR spectroscopy, characterize two mechanisms by which the RPA/p53 interaction can be modulated. RPA70N forms an oligonucleotide/oligosaccharide-binding fold, similar to that previously observed for the ssDNA-binding domains of RPA. In contrast, the N-terminal p53 transactivation domain is largely disordered in solution, but residues 37-57 fold into two amphipathic helices, H1 and H2, upon binding with RPA70N. The H2 helix of p53 structurally mimics the binding of ssDNA to the oligonucleotide/oligosaccharide-binding fold. NMR experiments confirmed that both ssDNA and an acidic peptide mimicking a phosphorylated form of RPA32N can independently compete the acidic p53N out of the binding site. Taken together, our data suggest a mechanism for DNA damage signaling that can explain a threshold response to DNA damage.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16234232}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16234232 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16234232}}
==About this Structure==
==About this Structure==
Line 30: Line 34:
[[Category: Okorokov, A.]]
[[Category: Okorokov, A.]]
[[Category: Replication]]
[[Category: Replication]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:45:39 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:58:05 2008''

Revision as of 06:58, 29 July 2008

Image:2b29.png

Template:STRUCTURE 2b29

N-terminal domain of the RPA70 subunit of human replication protein A.

Template:ABSTRACT PUBMED 16234232

About this Structure

2B29 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Single-stranded DNA mimicry in the p53 transactivation domain interaction with replication protein A., Bochkareva E, Kaustov L, Ayed A, Yi GS, Lu Y, Pineda-Lucena A, Liao JC, Okorokov AL, Milner J, Arrowsmith CH, Bochkarev A, Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15412-7. Epub 2005 Oct 17. PMID:16234232

Page seeded by OCA on Tue Jul 29 09:58:05 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2b29"
Personal tools