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2b2k

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[[Image:2b2k.jpg|left|200px]]
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{{STRUCTURE_2b2k| PDB=2b2k | SCENE= }}
{{STRUCTURE_2b2k| PDB=2b2k | SCENE= }}
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'''structure of Y104F IDI-1 mutant in complex with EIPP'''
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===structure of Y104F IDI-1 mutant in complex with EIPP===
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==Overview==
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Isopentenyl-diphosphate (IPP):dimethylallyl diphosphate isomerase is a key enzyme in the biosynthesis of isoprenoids. The mechanism of the isomerization reaction involves protonation of the unactivated carbon-carbon double bond in the substrate, but identity of the acidic moiety providing the proton is still not clear. Multiple sequence alignments and geometrical features observed in crystal structures of complexes with IPP isomerase suggest that Tyr-104 could play an important role during catalysis. A series of mutants was constructed by directed mutagenesis and characterized by enzymology. Crystallographic and thermal denaturation data for Y104A and Y104F mutants were obtained. Those data demonstrate the importance of residue Tyr-104 for proper folding of Escherichia coli type I IPP isomerase.
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{{ABSTRACT_PUBMED_16617181}}
==About this Structure==
==About this Structure==
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[[Category: Wouters, J.]]
[[Category: Wouters, J.]]
[[Category: Complex]]
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Revision as of 16:13, 28 July 2008

Image:2b2k.png

Template:STRUCTURE 2b2k

structure of Y104F IDI-1 mutant in complex with EIPP

Template:ABSTRACT PUBMED 16617181

About this Structure

2B2K is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural role for Tyr-104 in Escherichia coli isopentenyl-diphosphate isomerase: site-directed mutagenesis, enzymology, and protein crystallography., de Ruyck J, Durisotti V, Oudjama Y, Wouters J, J Biol Chem. 2006 Jun 30;281(26):17864-9. Epub 2006 Apr 15. PMID:16617181

Page seeded by OCA on Mon Jul 28 19:13:18 2008

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