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2b38

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{{STRUCTURE_2b38| PDB=2b38 | SCENE= }}
{{STRUCTURE_2b38| PDB=2b38 | SCENE= }}
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'''Solution structure of kalata B8'''
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===Solution structure of kalata B8===
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==Overview==
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The cyclotides are a family of circular proteins with a range of biological activities and potential pharmaceutical and agricultural applications. The biosynthetic mechanism of cyclization is unknown and the discovery of novel sequences may assist in achieving this goal. In the present study, we have isolated a new cyclotide from Oldenlandia affinis, kalata B8, which appears to be a hybrid of the two major subfamilies (Mobius and bracelet) of currently known cyclotides. We have determined the three-dimensional structure of kalata B8 and observed broadening of resonances directly involved in the cystine knot motif, suggesting flexibility in this region despite it being the core structural element of the cyclotides. The cystine knot motif is widespread throughout Nature and inherently stable, making this apparent flexibility a surprising result. Furthermore, there appears to be isomerization of the peptide backbone at an Asp-Gly sequence in the region involved in the cyclization process. Interestingly, such isomerization has been previously characterized in related cyclic knottins from Momordica cochinchinensis that have no sequence similarity to kalata B8 apart from the six conserved cysteine residues and may result from a common mechanism of cyclization. Kalata B8 also provides insight into the structure-activity relationships of cyclotides as it displays anti-HIV activity but lacks haemolytic activity. The 'uncoupling' of these two activities has not previously been observed for the cyclotides and may be related to the unusual hydrophilic nature of the peptide.
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(as it appears on PubMed at http://www.pubmed.gov), where 16207177 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16207177}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B38 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B38 OCA].
==Reference==
==Reference==
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[[Category: Cyclic backbone]]
[[Category: Cyclic backbone]]
[[Category: Cystine knot]]
[[Category: Cystine knot]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:47:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:44:57 2008''

Revision as of 04:45, 29 July 2008

Image:2b38.png

Template:STRUCTURE 2b38

Solution structure of kalata B8

Template:ABSTRACT PUBMED 16207177

About this Structure

Full experimental information is available from OCA.

Reference

Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif., Daly NL, Clark RJ, Plan MR, Craik DJ, Biochem J. 2006 Feb 1;393(Pt 3):619-26. PMID:16207177

Page seeded by OCA on Tue Jul 29 07:44:57 2008

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