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2b4u

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[[Image:2b4u.gif|left|200px]]
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{{STRUCTURE_2b4u| PDB=2b4u | SCENE= }}
{{STRUCTURE_2b4u| PDB=2b4u | SCENE= }}
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'''Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase'''
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===Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase===
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==Overview==
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PhyA from Selenomonas ruminantium (PhyAsr), is a bacterial protein tyrosine phosphatase (PTP)-like inositol polyphosphate phosphatase (IPPase) that is distantly related to known PTPs. PhyAsr has a second substrate binding site referred to as a standby site and the P-loop (HCX5R) has been observed in both open (inactive) and closed (active) conformations. Site-directed mutagenesis and kinetic and structural studies indicate PhyAsr follows a classical PTP mechanism of hydrolysis and has a broad specificity toward polyphosphorylated myo-inositol substrates, including phosphoinositides. Kinetic and molecular docking experiments demonstrate PhyAsr preferentially cleaves the 3-phosphate position of Ins P6 and will produce Ins(2)P via a highly ordered series of sequential dephosphorylations: D-Ins(1,2,4,5,6)P5, Ins(2,4,5,6)P4, D-Ins(2,4,5)P3, and D-Ins(2,4)P2. The data support a distributive enzyme mechanism and suggest the PhyAsr standby site is involved in the recruitment of substrate. Structural studies at physiological pH and high salt concentrations demonstrate the "closed" or active P-loop conformation can be induced in the absence of substrate. These results suggest PhyAsr should be reclassified as a D-3 myo-inositol hexakisphosphate phosphohydrolase and suggest the PhyAsr reaction mechanism is more similar to that of PTPs than previously suspected.
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(as it appears on PubMed at http://www.pubmed.gov), where 17567745 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17567745}}
==About this Structure==
==About this Structure==
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[[Category: Ionic strength]]
[[Category: Ionic strength]]
[[Category: Ptp-like]]
[[Category: Ptp-like]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:51:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:16:24 2008''

Revision as of 03:16, 28 July 2008

Image:2b4u.png

Template:STRUCTURE 2b4u

Structure of the C252S mutant of Selenomonas ruminantium PTP-like phytase

Template:ABSTRACT PUBMED 17567745

About this Structure

2B4U is a Single protein structure of sequence from Selenomonas ruminantium. Full crystallographic information is available from OCA.

Reference

Kinetic and structural analysis of a bacterial protein tyrosine phosphatase-like myo-inositol polyphosphatase., Puhl AA, Gruninger RJ, Greiner R, Janzen TW, Mosimann SC, Selinger LB, Protein Sci. 2007 Jul;16(7):1368-78. Epub 2007 Jun 13. PMID:17567745

Page seeded by OCA on Mon Jul 28 06:16:24 2008

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