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| - | [[Image:2b7j.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2b7j| PDB=2b7j | SCENE= }} | | {{STRUCTURE_2b7j| PDB=2b7j | SCENE= }} |
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| - | '''Crystal Structure of Yeast Sco1 with Copper Bound'''
| + | ===Crystal Structure of Yeast Sco1 with Copper Bound=== |
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| - | ==Overview==
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| - | The Sco family of proteins are involved in the assembly of the dinuclear CuA site in cytochrome c oxidase (COX), the terminal enzyme in aerobic respiration. These proteins, which are found in both eukaryotes and prokaryotes, are characterized by a conserved CXXXC sequence motif that binds copper ions and that has also been proposed to perform a thiol:disulfide oxidoreductase function. The crystal structures of Saccharomyces cerevisiae apo Sco1 (apo-ySco1) and Sco1 in the presence of copper ions (Cu-ySco1) were determined to 1.8- and 2.3-A resolutions, respectively. Yeast Sco1 exhibits a thioredoxin-like fold, similar to that observed for human Sco1 and a homolog from Bacillus subtilis. The Cu-ySco1 structure, obtained by soaking apo-ySco1 crystals in copper ions, reveals an unexpected copper-binding site involving Cys181 and Cys216, cysteine residues present in ySco1 but not in other homologs. The conserved CXXXC cysteines, Cys148 and Cys152, can undergo redox chemistry in the crystal. An essential histidine residue, His239, is located on a highly flexible loop, denoted the Sco loop, and can adopt positions proximal to both pairs of cysteines. Interactions between ySco1 and its partner proteins yeast Cox17 and yeast COX2 are likely to occur via complementary electrostatic surfaces. This high-resolution model of a eukaryotic Sco protein provides new insight into Sco copper binding and function. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16570183}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16570183 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16570183}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Sco]] | | [[Category: Sco]] |
| | [[Category: Sco1]] | | [[Category: Sco1]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:57:09 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:41:20 2008'' |
Revision as of 17:41, 27 July 2008
Template:STRUCTURE 2b7j
Crystal Structure of Yeast Sco1 with Copper Bound
Template:ABSTRACT PUBMED 16570183
About this Structure
2B7J is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Crystal structure of yeast Sco1., Abajian C, Rosenzweig AC, J Biol Inorg Chem. 2006 Jun;11(4):459-66. Epub 2006 Mar 29. PMID:16570183
Page seeded by OCA on Sun Jul 27 20:41:20 2008
