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| - | [[Image:2b7x.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2b7x| PDB=2b7x | SCENE= }} | | {{STRUCTURE_2b7x| PDB=2b7x | SCENE= }} |
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| - | '''Sequential reorganization of beta-sheet topology by insertion of a single strand'''
| + | ===Sequential reorganization of beta-sheet topology by insertion of a single strand=== |
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| - | ==Overview==
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| - | Insertions, duplications, and deletions of sequence segments are thought to be major evolutionary mechanisms that increase the structural and functional diversity of proteins. Alternative splicing, for example, is an intracellular editing mechanism that is thought to generate isoforms for 30%-50% of all human genes. Whereas the inserted sequences usually display only minor structural rearrangements at the insertion site, recent observations indicate that they may also cause more dramatic structural displacements of adjacent structures. In the present study we test how artificially inserted sequences change the structure of the beta-sheet region in T4 lysozyme. Copies of two different beta-strands were inserted into two different loops of the beta-sheet, and the structures were determined. Not surprisingly, one insert "loops out" at its insertion site and forms a new small beta-hairpin structure. Unexpectedly, however, the second insertion leads to displacement of adjacent strands and a sequential reorganization of the beta-sheet topology. Even though the insertions were performed at two different sites, looping out occurred at the C-terminal end of the same beta-strand. Reasons as to why a non-native sequence would be recruited to replace that which occurs in the native protein are discussed. Our results illustrate how sequence insertions can facilitate protein evolution through both local and nonlocal changes in structure.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16597830}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16597830 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16597830}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Structural switch]] | | [[Category: Structural switch]] |
| | [[Category: Tandem repeat]] | | [[Category: Tandem repeat]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 19:58:00 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:51:17 2008'' |
Revision as of 12:51, 28 July 2008
Template:STRUCTURE 2b7x
Sequential reorganization of beta-sheet topology by insertion of a single strand
Template:ABSTRACT PUBMED 16597830
About this Structure
2B7X is a Single protein structure of sequence from Enterobacteria phage t4. Full crystallographic information is available from OCA.
Reference
Sequential reorganization of beta-sheet topology by insertion of a single strand., Sagermann M, Baase WA, Matthews BW, Protein Sci. 2006 May;15(5):1085-92. Epub 2006 Apr 5. PMID:16597830
Page seeded by OCA on Mon Jul 28 15:51:17 2008
