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2be7

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{{STRUCTURE_2be7| PDB=2be7 | SCENE= }}
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'''Crystal structure of the unliganded (T-state) aspartate transcarbamoylase of the psychrophilic bacterium Moritella profunda'''
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===Crystal structure of the unliganded (T-state) aspartate transcarbamoylase of the psychrophilic bacterium Moritella profunda===
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==Overview==
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Aspartate carbamoyltransferase (EC 2.1.3.2) is extensively studied as a model for cooperativity and allosteric regulation. The structure of the Escherichia coli enzyme has been thoroughly analyzed by X-ray crystallography, and recently the crystal structures of two hyperthermophilic ATCases of the same structural class have been characterized. We here report the detailed functional and structural investigation of the ATCase from the psychrophilic deep sea bacterium Moritella profunda. Our analysis indicates that the enzyme conforms to the E. coli model in that two allosteric states exist that are influenced by similar homotropic interactions. The heterotropic properties differ in that CTP and UTP inhibit the holoenzyme, but ATP seems to exhibit a dual regulatory pattern, activating the enzyme at low concentrations and inhibiting it in the mM range. The crystal structure of the unliganded M. profunda ATCase shows resemblance to a more extreme T state reported previously for an E. coli ATCase mutant. A detailed molecular analysis reveals potential features of adaptation to cold activity and cold regulation. Moreover, M. profunda ATCase presents similarities with certain mutants of E. coli ATCase altered in their kinetic properties or temperature relationships. Finally, structural and functional comparison of ATCases across the full physiological temperature range agrees with an important, but fundamentally different role for electrostatics in protein adaptation at both extremes, i.e. an increased stability through the formation of ion pairs and ion pair networks at high physiological temperatures, and an increased flexibility through enhanced protein solvation at low temperatures.
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==About this Structure==
==About this Structure==
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[[Category: Psychrophilic]]
[[Category: Psychrophilic]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 07:16, 29 July 2008

Image:2be7.png

Template:STRUCTURE 2be7

Crystal structure of the unliganded (T-state) aspartate transcarbamoylase of the psychrophilic bacterium Moritella profunda

Template:ABSTRACT PUBMED 17070547

About this Structure

2BE7 is a Protein complex structure of sequences from Moritella profunda. Full crystallographic information is available from OCA.

Reference

Structural investigation of cold activity and regulation of aspartate carbamoyltransferase from the extreme psychrophilic bacterium Moritella profunda., De Vos D, Xu Y, Hulpiau P, Vergauwen B, Van Beeumen JJ, J Mol Biol. 2007 Jan 12;365(2):379-95. Epub 2006 Sep 29. PMID:17070547

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