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2bgp

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[[Image:2bgp.gif|left|200px]]
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{{STRUCTURE_2bgp| PDB=2bgp | SCENE= }}
{{STRUCTURE_2bgp| PDB=2bgp | SCENE= }}
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'''MANNAN BINDING MODULE FROM MAN5C IN BOUND CONFORMATION'''
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===MANNAN BINDING MODULE FROM MAN5C IN BOUND CONFORMATION===
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==Overview==
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Enzymes that digest plant cell wall polysaccharides generally contain non-catalytic, carbohydrate-binding modules (CBMs) that function by attaching the enzyme to the substrate, potentiating catalytic activity. Here, we present the first structure of a family 35 CBM, derived from the Cellvibrio japonicus beta-1,4-mannanase Man5C. The NMR structure has been determined for both the free protein and the protein bound to mannopentaose. The data show that the protein displays a typical beta-jelly-roll fold. Ligand binding is not located on the concave surface of the protein, as occurs in many CBMs that display the jelly-roll fold, but is formed by the loops that link the two beta-sheets of the protein, similar to family 6 CBMs. In contrast to the majority of CBMs, which are generally rigid proteins, CBM35 undergoes significant conformational change upon ligand binding. The curvature of the binding site and the narrow binding cleft are likely to be the main determinants of binding specificity. The predicted solvent exposure of O6 at several subsites provides an explanation for the observed accommodation of decorated mannans. Two of the key aromatic residues in Man5C-CBM35 that interact with mannopentaose are conserved in mannanase-derived CBM35s, which will guide specificity predictions based on the primary sequence of proteins in this CBM family.
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{{ABSTRACT_PUBMED_15740741}}
==About this Structure==
==About this Structure==
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2BGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGP OCA].
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2BGP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Cellvibrio_japonicus Cellvibrio japonicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BGP OCA].
==Reference==
==Reference==
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[[Category: Carbohydrate binding module]]
[[Category: Carbohydrate binding module]]
[[Category: Mannan]]
[[Category: Mannan]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:15:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:29:47 2008''

Revision as of 12:29, 28 July 2008

Image:2bgp.png

Template:STRUCTURE 2bgp

MANNAN BINDING MODULE FROM MAN5C IN BOUND CONFORMATION

Template:ABSTRACT PUBMED 15740741

About this Structure

2BGP is a Single protein structure of sequence from Cellvibrio japonicus. Full experimental information is available from OCA.

Reference

Structure of a mannan-specific family 35 carbohydrate-binding module: evidence for significant conformational changes upon ligand binding., Tunnicliffe RB, Bolam DN, Pell G, Gilbert HJ, Williamson MP, J Mol Biol. 2005 Mar 25;347(2):287-96. PMID:15740741

Page seeded by OCA on Mon Jul 28 15:29:47 2008

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