2bh2

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{{STRUCTURE_2bh2| PDB=2bh2 | SCENE= }}
{{STRUCTURE_2bh2| PDB=2bh2 | SCENE= }}
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'''CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.'''
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===CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.===
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==Overview==
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A single base (U1939) within E. coli 23S ribosomal RNA is methylated by its dedicated enzyme, RumA. The structure of RumA/RNA/S-adenosylhomocysteine uncovers the mechanism for achieving unique selectivity. The single-stranded substrate is "refolded" on the enzyme into a compact conformation with six key intra-RNA interactions. The RNA substrate contributes directly to catalysis. In addition to the target base, a second base is "flipped out" from the core loop to stack against the adenine of the cofactor S-adenosylhomocysteine. Nucleotides in permuted sequence order are stacked into the site vacated by the everted target U1939 and compensate for the energetic penalty of base eversion. The 3' hairpin segment of the RNA binds distal to the active site and provides binding energy that contributes to enhanced catalytic efficiency. Active collaboration of RNA in catalysis leads us to conclude that RumA and its substrate RNA may reflect features from the earliest RNA-protein era.
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(as it appears on PubMed at http://www.pubmed.gov), where 15766524 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15766524}}
==About this Structure==
==About this Structure==
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[[Category: Substrate selectivity]]
[[Category: Substrate selectivity]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:16:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 00:51:21 2008''

Revision as of 21:51, 27 July 2008

Image:2bh2.png

Template:STRUCTURE 2bh2

CRYSTAL STRUCTURE OF E. COLI 5-METHYLURIDINE METHYLTRANSFERASE RUMA IN COMPLEX WITH RIBOSOMAL RNA SUBSTRATE AND S-ADENOSYLHOMOCYSTEINE.

Template:ABSTRACT PUBMED 15766524

About this Structure

2BH2 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function., Lee TT, Agarwalla S, Stroud RM, Cell. 2005 Mar 11;120(5):599-611. PMID:15766524

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