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2bjm

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[[Image:2bjm.gif|left|200px]]
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{{Seed}}
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[[Image:2bjm.png|left|200px]]
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{{STRUCTURE_2bjm| PDB=2bjm | SCENE= }}
{{STRUCTURE_2bjm| PDB=2bjm | SCENE= }}
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'''SPE7:ANTHRONE COMPLEX'''
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===SPE7:ANTHRONE COMPLEX===
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==Overview==
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Induced fit is a predominant phenomenon in protein-ligand interactions, yet it is invariably attributed without establishing the existence, let alone the structure, of the initial, low-affinity encounter complex. We determined the crystal structure of the encounter complex on the pathway of ligand binding by IgE antibody SPE7. We show that this complex is formed by a wide range of ligands that initially bind with identical affinity. Nonspecific ligands rapidly dissociate, whereupon the antibody isomerizes to a nonbinding isomer. Specific ligand complexes, however, slowly isomerize to give a high-affinity complex. This isomerization involves backbone and side-chain rearrangements of up to 14 A and the formation of specific hydrogen bonds. The postbinding conformational switch, combined with the prebinding isomerization to an energetically favorable nonbinding isomer, results in a "kinetic discrimination" mechanism that mediates selective binding, by a factor of &gt;10(3), between highly related ligands that initially bind with the same affinity. This model may apply to proteins that bind multiple ligands in a specific manner or other proteins that, although capable of binding many ligands, are activated by only a few.
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The line below this paragraph, {{ABSTRACT_PUBMED_16129832}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16129832 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16129832}}
==About this Structure==
==About this Structure==
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[[Category: Multispecificity]]
[[Category: Multispecificity]]
[[Category: Promiscuity]]
[[Category: Promiscuity]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:22:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 23:04:03 2008''

Revision as of 20:04, 28 July 2008

Image:2bjm.png

Template:STRUCTURE 2bjm

SPE7:ANTHRONE COMPLEX

Template:ABSTRACT PUBMED 16129832

About this Structure

2BJM is a Single protein structure of sequence from Rattus rattus. Full crystallographic information is available from OCA.

Reference

Structure and kinetics of a transient antibody binding intermediate reveal a kinetic discrimination mechanism in antigen recognition., James LC, Tawfik DS, Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12730-5. Epub 2005 Aug 29. PMID:16129832

Page seeded by OCA on Mon Jul 28 23:04:03 2008

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