From Proteopedia
(Difference between revisions)
proteopedia linkproteopedia link
|
|
| Line 1: |
Line 1: |
| - | [[Image:2bjr.gif|left|200px]] | + | {{Seed}} |
| | + | [[Image:2bjr.png|left|200px]] |
| | | | |
| | <!-- | | <!-- |
| Line 9: |
Line 10: |
| | {{STRUCTURE_2bjr| PDB=2bjr | SCENE= }} | | {{STRUCTURE_2bjr| PDB=2bjr | SCENE= }} |
| | | | |
| - | '''CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2B'''
| + | ===CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2B=== |
| | | | |
| | | | |
| - | ==Overview==
| + | <!-- |
| - | The simplicity and specialization of the cell motility machinery of Ascaris sperm provides a powerful system in which to probe the basic molecular mechanism of amoeboid cell motility. Although Ascaris sperm locomotion closely resembles that seen in many other types of crawling cell, movement is generated by modulation of a cytoskeleton based on the major sperm protein (MSP) rather than the actin present in other cell types. The Ascaris motility machinery can be studied conveniently in a cell-free in vitro system based on the movement of plasma membrane vesicles by fibres constructed from bundles of MSP filaments. In addition to ATP, MSP and a plasma membrane protein, reconstitution of MSP motility in this cell-free extract requires cytosolic proteins to orchestrate the site-specific assembly and bundling of MSP filaments that generates locomotion. One of these proteins, MFP2, accelerates the rate of movement in this assay. Here, we describe crystal structures of two isoforms of MFP2 and show that both are constructed from two domains that have the same fold based on a novel, compact beta sheet arrangement. Patterns of conservation observed in a structure-based analysis of MFP2 sequences from different nematode species identified regions that may be putative functional interfaces involved both in interactions between MFP2 domains and also with other components of the sperm motility machinery. Analysis of the growth of fibres in vitro in the presence of added MFP2 indicated that MFP2 increases the rate of locomotion by enhancing the effective rate of MSP filament polymerization. This observation, together with the structural data, suggests that MFP2 may function in a manner analogous to formins in actin-based motility. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15755452}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15755452 is the PubMed ID number. |
| | + | --> |
| | + | {{ABSTRACT_PUBMED_15755452}} |
| | | | |
| | ==About this Structure== | | ==About this Structure== |
| Line 31: |
Line 35: |
| | [[Category: Msp]] | | [[Category: Msp]] |
| | [[Category: Nematode]] | | [[Category: Nematode]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:23:03 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 18:22:14 2008'' |
Revision as of 15:22, 27 July 2008
Template:STRUCTURE 2bjr
CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2B
Template:ABSTRACT PUBMED 15755452
About this Structure
2BJR is a Single protein structure of sequence from Ascaris suum. Full crystallographic information is available from OCA.
Reference
Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility., Grant RP, Buttery SM, Ekman GC, Roberts TM, Stewart M, J Mol Biol. 2005 Apr 1;347(3):583-95. PMID:15755452
Page seeded by OCA on Sun Jul 27 18:22:14 2008
