2bku

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[[Image:2bku.gif|left|200px]]
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[[Image:2bku.png|left|200px]]
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==Overview==
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{{ABSTRACT_15864302}}
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Nuclear protein import is mediated mainly by the transport factor importin-beta that binds cytoplasmic cargo, most often via the importin-alpha adaptor, and then transports it through nuclear pore complexes. This active transport is driven by disassembly of the import complex by nuclear RanGTP. The switch I and II loops of Ran change conformation with nucleotide state, and regulate its interactions with nuclear trafficking components. Importin-beta consists of 19 HEAT repeats that are based on a pair of antiparallel alpha-helices (referred to as the A- and B-helices). The HEAT repeats stack to yield two C-shaped arches, linked together to form a helicoidal molecule that has considerable conformational flexibility. Here we present the structure of full-length yeast importin-beta (Kap95p or karyopherin-beta) complexed with RanGTP, which provides a basis for understanding the crucial cargo-release step of nuclear import. We identify a key interaction site where the RanGTP switch I loop binds to the carboxy-terminal arch of Kap95p. This interaction produces a change in helicoidal pitch that locks Kap95p in a conformation that cannot bind importin-alpha or cargo. We suggest an allosteric mechanism for nuclear import complex disassembly by RanGTP.
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==About this Structure==
==About this Structure==
2BKU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 2BKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKU OCA].
2BKU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 2BKU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BKU OCA].
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==Reference==
 
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Structural basis for nuclear import complex dissociation by RanGTP., Lee SJ, Matsuura Y, Liu SM, Stewart M, Nature. 2005 Jun 2;435(7042):693-6. Epub 2005 May 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15864302 15864302]
 
[[Category: Canis lupus familiaris]]
[[Category: Canis lupus familiaris]]
[[Category: Importins]]
[[Category: Importins]]
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[[Category: Protein transport]]
[[Category: Protein transport]]
[[Category: Rangtp]]
[[Category: Rangtp]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:25:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:13:27 2008''

Revision as of 15:13, 26 June 2008

Image:2bku.png

Template:STRUCTURE 2bku

KAP95P:RANGTP COMPLEX


Template:ABSTRACT 15864302

About this Structure

2BKU is a Protein complex structure of sequences from Canis lupus familiaris and Saccharomyces cerevisiae. The following page contains interesting information on the relation of 2BKU with [Importins]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:13:27 2008

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