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| - | [[Image:2bl0.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2bl0| PDB=2bl0 | SCENE= }} | | {{STRUCTURE_2bl0| PDB=2bl0 | SCENE= }} |
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| - | '''PHYSARUM POLYCEPHALUM MYOSIN II REGULATORY DOMAIN'''
| + | ===PHYSARUM POLYCEPHALUM MYOSIN II REGULATORY DOMAIN=== |
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| - | ==Overview==
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| - | We have previously identified a single inhibitory Ca2+-binding site in the first EF-hand of the essential light chain of Physarum conventional myosin (Farkas, L., Malnasi-Csizmadia, A., Nakamura, A., Kohama, K., and Nyitray, L. (2003) J. Biol. Chem. 278, 27399-27405). As a general rule, conformation of the EF-hand-containing domains in the calmodulin family is "closed" in the absence and "open" in the presence of bound cations; a notable exception is the unusual Ca2+-bound closed domain in the essential light chain of the Ca2+-activated scallop muscle myosin. Here we have reported the 1.8 A resolution structure of the regulatory domain (RD) of Physarum myosin II in which Ca2+ is bound to a canonical EF-hand that is also in a closed state. The 12th position of the EF-hand loop, which normally provides a bidentate ligand for Ca2+ in the open state, is too far in the structure to participate in coordination of the ion. The structure includes a second Ca2+ that only mediates crystal contacts. To reveal the mechanism behind the regulatory effect of Ca2+, we compared conformational flexibilities of the liganded and unliganded RD. Our working hypothesis, i.e. the modulatory effect of Ca2+ on conformational flexibility of RD, is in line with the observed suppression of hydrogen-deuterium exchange rate in the Ca2+-bound form, as well as with results of molecular dynamics calculations. Based on this evidence, we concluded that Ca2+-induced change in structural dynamics of RD is a major factor in Ca2+-mediated regulation of Physarum myosin II activity.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16227209}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16227209 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16227209}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Myosin]] | | [[Category: Myosin]] |
| | [[Category: Slime mould]] | | [[Category: Slime mould]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:26:10 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 16:27:36 2008'' |
Revision as of 13:27, 27 July 2008
Template:STRUCTURE 2bl0
PHYSARUM POLYCEPHALUM MYOSIN II REGULATORY DOMAIN
Template:ABSTRACT PUBMED 16227209
About this Structure
2BL0 is a Protein complex structure of sequences from Physarum polycephalum. Full crystallographic information is available from OCA.
Reference
Structural evidence for non-canonical binding of Ca2+ to a canonical EF-hand of a conventional myosin., Debreczeni JE, Farkas L, Harmat V, Hetenyi C, Hajdu I, Zavodszky P, Kohama K, Nyitray L, J Biol Chem. 2005 Dec 16;280(50):41458-64. Epub 2005 Oct 13. PMID:16227209
Page seeded by OCA on Sun Jul 27 16:27:36 2008
