2f8y
From Proteopedia
(New page: 200px<br /> <applet load="2f8y" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f8y, resolution 1.55Å" /> '''Crystal structure o...) |
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| - | [[Image:2f8y.gif|left|200px]]<br /> | + | [[Image:2f8y.gif|left|200px]]<br /><applet load="2f8y" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2f8y" size=" | + | |
caption="2f8y, resolution 1.55Å" /> | caption="2f8y, resolution 1.55Å" /> | ||
'''Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution.'''<br /> | '''Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Notch receptors transduce essential developmental signals between | + | Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2F8Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2F8Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F8Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blacklow, S | + | [[Category: Blacklow, S C.]] |
[[Category: Nam, Y.]] | [[Category: Nam, Y.]] | ||
[[Category: Sliz, P.]] | [[Category: Sliz, P.]] | ||
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[[Category: notch]] | [[Category: notch]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:18:57 2008'' |
Revision as of 15:18, 21 February 2008
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Crystal structure of human Notch1 ankyrin repeats to 1.55A resolution.
Contents |
Overview
Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.
Disease
Known diseases associated with this structure: Aortic valve disease OMIM:[190198], Leukemia, T-cell acute lymphoblastic OMIM:[190198]
About this Structure
2F8Y is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes., Nam Y, Sliz P, Song L, Aster JC, Blacklow SC, Cell. 2006 Mar 10;124(5):973-83. PMID:16530044
Page seeded by OCA on Thu Feb 21 17:18:57 2008
Categories: Homo sapiens | Single protein | Blacklow, S C. | Nam, Y. | Sliz, P. | SO4 | Ankyrin repeats | Notch
