2f9d
From Proteopedia
(New page: 200px<br /> <applet load="2f9d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2f9d, resolution 2.50Å" /> '''2.5 angstrom resolu...) |
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caption="2f9d, resolution 2.50Å" /> | caption="2f9d, resolution 2.50Å" /> | ||
'''2.5 angstrom resolution structure of the spliceosomal protein p14 bound to region of SF3b155'''<br /> | '''2.5 angstrom resolution structure of the spliceosomal protein p14 bound to region of SF3b155'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The precise excision of introns from precursor mRNAs (pre-mRNAs) in | + | The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome. |
==About this Structure== | ==About this Structure== | ||
| - | 2F9D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 2F9D is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F9D OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Edwards, R | + | [[Category: Edwards, R A.]] |
| - | [[Category: Glover, J | + | [[Category: Glover, J N.M.]] |
| - | [[Category: Macmillan, A | + | [[Category: Macmillan, A M.]] |
| - | [[Category: Ritchie, D | + | [[Category: Ritchie, D B.]] |
| - | [[Category: Schellenberg, M | + | [[Category: Schellenberg, M J.]] |
[[Category: p14 sf3bp14 sf3b155 sap155 rrm]] | [[Category: p14 sf3bp14 sf3b155 sap155 rrm]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:19:04 2008'' |
Revision as of 15:19, 21 February 2008
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2.5 angstrom resolution structure of the spliceosomal protein p14 bound to region of SF3b155
Overview
The precise excision of introns from precursor mRNAs (pre-mRNAs) in eukaryotes is accomplished by the spliceosome, a complex assembly containing five small nuclear ribonucleoprotein (snRNP) particles. Human p14, a component of the spliceosomal U2 and U11/U12 snRNPs, has been shown to associate directly with the pre-mRNA branch adenosine early in spliceosome assembly and within the fully assembled spliceosome. Here we report the 2.5-A crystal structure of a complex containing p14 and a peptide derived from the p14-associated U2 snRNP component SF3b155. p14 contains an RNA recognition motif (RRM), the surface of which is largely occluded by a C-terminal alpha-helix and a portion of the SF3b155 peptide. An analysis of RNA.protein crosslinking to wild-type and mutant p14 shows that the branch adenosine directly interacts with a conserved aromatic within a pocket on the surface of the complex. This result, combined with a comparison of the structure with known RRMs and pseudoRRMs as well as model-building by using the electron cryomicroscopy structure of a spliceosomal U11/U12 di-snRNP, suggests that p14.SF3b155 presents a noncanonical surface for RNA recognition at the heart of the mammalian spliceosome.
About this Structure
2F9D is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of a core spliceosomal protein interface., Schellenberg MJ, Edwards RA, Ritchie DB, Kent OA, Golas MM, Stark H, Luhrmann R, Glover JN, MacMillan AM, Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1266-71. Epub 2006 Jan 23. PMID:16432215
Page seeded by OCA on Thu Feb 21 17:19:04 2008
