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2bsi

From Proteopedia

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{{STRUCTURE_2bsi| PDB=2bsi | SCENE= }}
{{STRUCTURE_2bsi| PDB=2bsi | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE TYPE III SECRETION CHAPERONE SYCT FROM YERSINIA ENTEROCOLITICA (CRYSTAL FORM 1)'''
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===CRYSTAL STRUCTURE OF THE TYPE III SECRETION CHAPERONE SYCT FROM YERSINIA ENTEROCOLITICA (CRYSTAL FORM 1)===
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==Overview==
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Pathogenic Yersinia species use a type III secretion (TTS) system to deliver a number of cytotoxic effector proteins directly into the mammalian host cell. To ensure effective translocation, several such effector proteins transiently bind to specific chaperones in the bacterial cytoplasm. Correspondingly, SycT is the chaperone of YopT, a cysteine protease that cleaves the membrane-anchor of Rho-GTPases in the host. We have analyzed the complex between YopT and SycT and determined the structure of SycT in three crystal forms. Biochemical studies indicate a stoichometric effector/chaperone ratio of 1:2 and the chaperone-binding site contains at least residues 52-103 of YopT. The crystal structures reveal a SycT homodimer with an overall fold similar to that of other TTS effector chaperones. In contrast to the canonical five-stranded anti-parallel beta-sheet flanked by three alpha-helices, SycT lacks the dimerization alpha-helix and has an additional beta-strand capable of undergoing a conformational change. The dimer interface consists of two beta-strands and the connecting loops. Two hydrophobic patches involved in effector binding in other TTS effector chaperones are also found in SycT. The structural similarity of SycT to other chaperones and the spatial conservation of effector-binding sites support the idea that TTS effector chaperones form a single functional and structural group.
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(as it appears on PubMed at http://www.pubmed.gov), where 16046625 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16046625}}
==About this Structure==
==About this Structure==
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[[Category: Yersinia]]
[[Category: Yersinia]]
[[Category: Yopt]]
[[Category: Yopt]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:36:52 2008''

Revision as of 06:36, 28 July 2008

Image:2bsi.png

Template:STRUCTURE 2bsi

CRYSTAL STRUCTURE OF THE TYPE III SECRETION CHAPERONE SYCT FROM YERSINIA ENTEROCOLITICA (CRYSTAL FORM 1)

Template:ABSTRACT PUBMED 16046625

About this Structure

2BSI is a Single protein structure of sequence from Yersinia enterocolitica. Full crystallographic information is available from OCA.

Reference

Crystal structure of Yersinia enterocolitica type III secretion chaperone SycT., Buttner CR, Cornelis GR, Heinz DW, Niemann HH, Protein Sci. 2005 Aug;14(8):1993-2002. PMID:16046625

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