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2bvc

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{{STRUCTURE_2bvc| PDB=2bvc | SCENE= }}
{{STRUCTURE_2bvc| PDB=2bvc | SCENE= }}
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'''CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A TRANSITION STATE MIMIC'''
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===CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A TRANSITION STATE MIMIC===
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==Overview==
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Glutamine synthetase catalyzes the ligation of glutamate and ammonia to form glutamine, with the resulting hydrolysis of ATP. The enzyme is a central component of bacterial nitrogen metabolism and is a potential drug target. Here, we report a high-yield recombinant expression system for glutamine synthetase of Mycobacterium tuberculosis together with a simple purification. The procedure allowed the structure of a complex with a phosphorylated form of the inhibitor methionine sulfoximine, magnesium, and ADP to be solved by molecular replacement and refined at 2.1-A resolution. To our knowledge, this study provides the first reported structure for a taut form of the M. tuberculosis enzyme, similar to that observed for the Salmonella enzyme earlier. The phospho compound, generated in situ by an active enzyme, mimics the phosphorylated tetrahedral adduct at the transition state. Some differences in ligand interactions of the protein with both phosphorylated compound and nucleotide are observed compared with earlier structures; a third metal ion also is found. The importance of these differences in the catalytic mechanism is discussed; the results will help guide the search for specific inhibitors of potential therapeutic interest.
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(as it appears on PubMed at http://www.pubmed.gov), where 16027359 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16027359}}
==About this Structure==
==About this Structure==
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[[Category: Synthetase]]
[[Category: Synthetase]]
[[Category: Transition state mimic]]
[[Category: Transition state mimic]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:51:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:58:21 2008''

Revision as of 07:58, 29 July 2008

Image:2bvc.png

Template:STRUCTURE 2bvc

CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS GLUTAMINE SYNTHETASE IN COMPLEX WITH A TRANSITION STATE MIMIC

Template:ABSTRACT PUBMED 16027359

About this Structure

2BVC is a Single protein structure of sequence from Mycobacterium tuberculosis. Full crystallographic information is available from OCA.

Reference

Structure of Mycobacterium tuberculosis glutamine synthetase in complex with a transition-state mimic provides functional insights., Krajewski WW, Jones TA, Mowbray SL, Proc Natl Acad Sci U S A. 2005 Jul 26;102(30):10499-504. Epub 2005 Jul 18. PMID:16027359

Page seeded by OCA on Tue Jul 29 10:58:21 2008

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