2iv0
From Proteopedia
(New page: 200px<br /> <applet load="2iv0" size="450" color="white" frame="true" align="right" spinBox="true" caption="2iv0, resolution 2.50Å" /> '''THERMAL STABILITY O...) |
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==About this Structure== | ==About this Structure== | ||
| - | 2IV0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]] with ZN and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IV0 OCA]]. | + | 2IV0 is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Archaeoglobus_fulgidus Archaeoglobus fulgidus]] with ZN and CL as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/Isocitrate_dehydrogenase_(NADP(+)) Isocitrate dehydrogenase (NADP(+))]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.42 1.1.1.42]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2IV0 OCA]]. |
==Reference== | ==Reference== | ||
Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers., Stokke R, Karlstrom M, Yang N, Leiros I, Ladenstein R, Birkeland NK, Steen IH, Extremophiles. 2007 May;11(3):481-93. Epub 2007 Mar 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17401542 17401542] | Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers., Stokke R, Karlstrom M, Yang N, Leiros I, Ladenstein R, Birkeland NK, Steen IH, Extremophiles. 2007 May;11(3):481-93. Epub 2007 Mar 31. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17401542 17401542] | ||
[[Category: Archaeoglobus fulgidus]] | [[Category: Archaeoglobus fulgidus]] | ||
| + | [[Category: Isocitrate dehydrogenase (NADP(+))]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Birkeland, N.K.]] | [[Category: Birkeland, N.K.]] | ||
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[[Category: tricarboxylic acid cycle]] | [[Category: tricarboxylic acid cycle]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:10:07 2007'' |
Revision as of 10:05, 30 October 2007
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THERMAL STABILITY OF ISOCITRATE DEHYDROGENASE FROM ARCHAEOGLOBUS FULGIDUS STUDIED BY CRYSTAL STRUCTURE ANALYSIS AND ENGINEERING OF CHIMERS
Overview
Isocitrate dehydrogenase from Archaeoglobus fulgidus (AfIDH) has an, apparent melting temperature (T (m)) of 98.5 degrees C. To identify the, structural features involved in thermal stabilization of AfIDH, the, structure was solved to 2.5 A resolution. AfIDH was strikingly similar to, mesophilic IDH from Escherichia coli (EcIDH) and displayed almost the same, number of ion pairs and ionic networks. However, two unique inter-domain, networks were present in AfIDH; one three-membered ionic network between, the large and the small domain and one four-membered ionic network between, the clasp and the small domain. The latter ionic network was presumably, reduced in size when the clasp domain of AfIDH was swapped with that of, EcIDH and the T (m) decreased by 18 degrees C. Contrarily, EcIDH ... [(full description)]
About this Structure
2IV0 is a [Single protein] structure of sequence from [Archaeoglobus fulgidus] with ZN and CL as [ligands]. Active as [Isocitrate dehydrogenase (NADP(+))], with EC number [1.1.1.42]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Thermal stability of isocitrate dehydrogenase from Archaeoglobus fulgidus studied by crystal structure analysis and engineering of chimers., Stokke R, Karlstrom M, Yang N, Leiros I, Ladenstein R, Birkeland NK, Steen IH, Extremophiles. 2007 May;11(3):481-93. Epub 2007 Mar 31. PMID:17401542
Page seeded by OCA on Tue Oct 30 12:10:07 2007
Categories: Archaeoglobus fulgidus | Isocitrate dehydrogenase (NADP(+)) | Single protein | Birkeland, N.K. | Karlstrom, M. | Ladenstein, R. | Leiros, I. | Steen, I.H. | Stokke, R. | Yang, N. | CL | ZN | Aromatic cluster | Domain swapping | Glyoxylate bypass | Ionic networks | Isocitrate dehydrogenase | Nadp | Oxidoreductase | Phosphorylation | Thermal stability | Tricarboxylic acid cycle
