2bym

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[[Image:2bym.gif|left|200px]]
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{{STRUCTURE_2bym| PDB=2bym | SCENE= }}
{{STRUCTURE_2bym| PDB=2bym | SCENE= }}
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'''HISTONE FOLD HETERODIMER OF THE CHROMATIN ACCESSIBILITY COMPLEX'''
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===HISTONE FOLD HETERODIMER OF THE CHROMATIN ACCESSIBILITY COMPLEX===
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==Overview==
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The chromatin accessibility complex (CHRAC) is an abundant, evolutionarily conserved nucleosome remodeling machinery able to catalyze histone octamer sliding on DNA. CHRAC differs from the related ACF complex by the presence of two subunits with molecular masses of 14 and 16 kDa, whose structure and function were not known. We determined the structure of Drosophila melanogaster CHRAC14-CHRAC16 by X-ray crystallography at 2.4-angstroms resolution and found that they dimerize via a variant histone fold in a typical handshake structure. In further analogy to histones, CHRAC14-16 contain unstructured N- and C-terminal tail domains that protrude from the handshake structure. A dimer of CHRAC14-16 can associate with the N terminus of ACF1, thereby completing CHRAC. Low-affinity interactions of CHRAC14-16 with DNA significantly improve the efficiency of nucleosome mobilization by limiting amounts of ACF. Deletion of the negatively charged C terminus of CHRAC16 enhances DNA binding 25-fold but leads to inhibition of nucleosome sliding, in striking analogy to the effect of the DNA chaperone HMGB1 on nucleosome sliding. The presence of a surface compatible with DNA interaction and the geometry of an H2A-H2B heterodimer may provide a transient acceptor site for DNA dislocated from the histone surface and therefore facilitate the nucleosome remodeling process.
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(as it appears on PubMed at http://www.pubmed.gov), where 16260604 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16260604}}
==About this Structure==
==About this Structure==
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[[Category: Histone fold]]
[[Category: Histone fold]]
[[Category: Nucleosome sliding]]
[[Category: Nucleosome sliding]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 20:58:51 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:58:36 2008''

Revision as of 21:58, 28 July 2008

Image:2bym.png

Template:STRUCTURE 2bym

HISTONE FOLD HETERODIMER OF THE CHROMATIN ACCESSIBILITY COMPLEX

Template:ABSTRACT PUBMED 16260604

About this Structure

2BYM is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.

Reference

The histone fold subunits of Drosophila CHRAC facilitate nucleosome sliding through dynamic DNA interactions., Hartlepp KF, Fernandez-Tornero C, Eberharter A, Grune T, Muller CW, Becker PB, Mol Cell Biol. 2005 Nov;25(22):9886-96. PMID:16260604

Page seeded by OCA on Tue Jul 29 00:58:36 2008

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