2c8o

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2c8o.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2c8o.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2c8o| PDB=2c8o | SCENE= }}
{{STRUCTURE_2c8o| PDB=2c8o | SCENE= }}
-
'''LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE'''
+
===LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE===
-
==Overview==
+
<!--
-
Structural proteomics has promoted the rapid development of automated protein structure determination using X-ray crystallography. Robotics are now routinely used along the pipeline from genes to protein structures. However, a bottleneck still remains. At synchrotron beamlines, the success rate of automated sample alignment along the X-ray beam is limited by difficulties in visualization of protein crystals, especially when they are small and embedded in mother liquor. Despite considerable improvement in optical microscopes, the use of visible light transmitted or reflected by the sample may result in poor or misleading contrast. Here, the endogenous fluorescence from aromatic amino acids has been used to identify even tiny or weakly fluorescent crystals with a high success rate. The use of a compact laser at 266 nm in combination with non-fluorescent sample holders provides an efficient solution to collect high-contrast fluorescence images in a few milliseconds and using standard camera optics. The best image quality was obtained with direct illumination through a viewing system coaxial with the UV beam. Crystallographic data suggest that the employed UV exposures do not generate detectable structural damage.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16510972}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16510972 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16510972}}
==About this Structure==
==About this Structure==
Line 38: Line 42:
[[Category: Uv]]
[[Category: Uv]]
[[Category: Visualisation]]
[[Category: Visualisation]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:27:18 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 00:48:25 2008''

Revision as of 21:48, 28 July 2008

Image:2c8o.png

Template:STRUCTURE 2c8o

LYSOZYME (1SEC) AND UV LASR EXCITED FLUORESCENCE

Template:ABSTRACT PUBMED 16510972

About this Structure

2C8O is a Single protein structure. Full crystallographic information is available from OCA.

Reference

UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:16510972

Page seeded by OCA on Tue Jul 29 00:48:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2c8o"
Personal tools