2c8q

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[[Image:2c8q.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_2c8q| PDB=2c8q | SCENE= }}
{{STRUCTURE_2c8q| PDB=2c8q | SCENE= }}
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'''INSULINE(1SEC) AND UV LASER EXCITED FLUORESCENCE'''
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===INSULINE(1SEC) AND UV LASER EXCITED FLUORESCENCE===
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==Overview==
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Structural proteomics has promoted the rapid development of automated protein structure determination using X-ray crystallography. Robotics are now routinely used along the pipeline from genes to protein structures. However, a bottleneck still remains. At synchrotron beamlines, the success rate of automated sample alignment along the X-ray beam is limited by difficulties in visualization of protein crystals, especially when they are small and embedded in mother liquor. Despite considerable improvement in optical microscopes, the use of visible light transmitted or reflected by the sample may result in poor or misleading contrast. Here, the endogenous fluorescence from aromatic amino acids has been used to identify even tiny or weakly fluorescent crystals with a high success rate. The use of a compact laser at 266 nm in combination with non-fluorescent sample holders provides an efficient solution to collect high-contrast fluorescence images in a few milliseconds and using standard camera optics. The best image quality was obtained with direct illumination through a viewing system coaxial with the UV beam. Crystallographic data suggest that the employed UV exposures do not generate detectable structural damage.
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The line below this paragraph, {{ABSTRACT_PUBMED_16510972}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16510972 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16510972}}
==About this Structure==
==About this Structure==
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:07:38 2008''

Revision as of 11:07, 28 July 2008

Image:2c8q.png

Template:STRUCTURE 2c8q

INSULINE(1SEC) AND UV LASER EXCITED FLUORESCENCE

Template:ABSTRACT PUBMED 16510972

About this Structure

2C8Q is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

UV laser-excited fluorescence as a tool for the visualization of protein crystals mounted in loops., Vernede X, Lavault B, Ohana J, Nurizzo D, Joly J, Jacquamet L, Felisaz F, Cipriani F, Bourgeois D, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):253-61. Epub 2006, Feb 22. PMID:16510972

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