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2c9i

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{{STRUCTURE_2c9i| PDB=2c9i | SCENE= }}
{{STRUCTURE_2c9i| PDB=2c9i | SCENE= }}
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'''STRUCTURE OF THE FLUORESCENT PROTEIN ASFP499 FROM ANEMONIA SULCATA'''
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===STRUCTURE OF THE FLUORESCENT PROTEIN ASFP499 FROM ANEMONIA SULCATA===
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==Overview==
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Despite their similar fold topologies, anthozoan fluorescent proteins (FPs) can exhibit widely different optical properties, arising either from chemical modification of the chromophore itself or from specific interactions of the chromophore with the surrounding protein moiety. Here we present a structural and spectroscopic investigation of the green FP asFP499 from the sea anemone Anemonia sulcata var. rufescens to explore the effects of the protein environment on the chromophore. The optical absorption and fluorescence spectra reveal two discrete species populated in significant proportions over a wide pH range. Moreover, multiple protonation reactions are evident from the observed pH-dependent spectral changes. The x-ray structure of asFP499, determined by molecular replacement at a resolution of 1.85 A, shows the typical beta-barrel fold of the green FP from Aequorea victoria (avGFP). In its center, the chromophore, formed from the tripeptide Gln(63)-Tyr(64)-Gly(65), is tightly held by multiple hydrogen bonds in a polar cage that is structurally quite dissimilar to that of avGFP. The x-ray structure provides interesting clues as to how the spectroscopic properties are fine tuned by the chromophore environment.
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{{ABSTRACT_PUBMED_16980366}}
==About this Structure==
==About this Structure==
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[[Category: Luminescence]]
[[Category: Luminescence]]
[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:30:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:16:17 2008''

Revision as of 14:16, 28 July 2008

Image:2c9i.png

Template:STRUCTURE 2c9i

STRUCTURE OF THE FLUORESCENT PROTEIN ASFP499 FROM ANEMONIA SULCATA

Template:ABSTRACT PUBMED 16980366

About this Structure

2C9I is a Single protein structure of sequence from Anemonia sulcata. Full crystallographic information is available from OCA.

Reference

Chromophore-protein interactions in the anthozoan green fluorescent protein asFP499., Nienhaus K, Renzi F, Vallone B, Wiedenmann J, Nienhaus GU, Biophys J. 2006 Dec 1;91(11):4210-20. Epub 2006 Sep 15. PMID:16980366

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