2ca6

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{{STRUCTURE_2ca6| PDB=2ca6 | SCENE= }}
{{STRUCTURE_2ca6| PDB=2ca6 | SCENE= }}
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'''MIRAS STRUCTURE DETERMINATION FROM HEMIHEDRALLY TWINNED CRYSTALS'''
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===MIRAS STRUCTURE DETERMINATION FROM HEMIHEDRALLY TWINNED CRYSTALS===
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==Overview==
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The structure of Rna1p was originally solved to 2.7 A resolution by MIRAS from crystals with partial hemihedral twinning in space group I4(1) [Hillig et al. (1999), Mol. Cell, 3, 781-791] by finding a low-twinned native crystal (twin fraction alpha=0.06) and after twin correction of all data sets. Rna1p crystals have now been used to examine how far twinning and twin correction affect MIR phasing with a higher resolution but highly twinned native data set. Even high hemihedral twinning [alphanative=0.39, alphaderivative=0.24] would not have hindered heavy-atom site identification of strong derivatives using difference Patterson maps. However, a weaker derivative could have been missed and refinement would have stalled at high R values had twinning not been identified and accounted for. Twin correction improved both site identification, experimental phasing statistics and MIR map quality. Different strategies were tested for refinement against twinned data. Using uncorrected twinned data and TWIN-CNS, Rna1p has now been refined to 2.2 A resolution (final twinned R and Rfree were 0.165 and 0.218, respectively). The increased resolution enabled release of the NCS restraints and allowed new conclusions to be drawn on the flexibility of the two molecules in the asymmetric unit. In the case of Rna1p, twinned crystal growth was possible owing to the presence of a twofold NCS axis almost parallel to the twin operator.
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(as it appears on PubMed at http://www.pubmed.gov), where 16790931 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16790931}}
==About this Structure==
==About this Structure==
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[[Category: Nuclear transport,signaling regulator]]
[[Category: Nuclear transport,signaling regulator]]
[[Category: Signaling activator]]
[[Category: Signaling activator]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:33:28 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:05:20 2008''

Revision as of 09:05, 29 July 2008

Image:2ca6.png

Template:STRUCTURE 2ca6

MIRAS STRUCTURE DETERMINATION FROM HEMIHEDRALLY TWINNED CRYSTALS

Template:ABSTRACT PUBMED 16790931

About this Structure

2CA6 is a Single protein structure of sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.

Reference

Detecting and overcoming hemihedral twinning during the MIR structure determination of Rna1p., Hillig RC, Renault L, Acta Crystallogr D Biol Crystallogr. 2006 Jul;62(Pt 7):750-65. Epub 2006, Jun 20. PMID:16790931

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