2fu5
From Proteopedia
(New page: 200px<br /> <applet load="2fu5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fu5, resolution 2.00Å" /> '''structure of Rab8 i...) |
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| - | [[Image:2fu5.gif|left|200px]]<br /> | + | [[Image:2fu5.gif|left|200px]]<br /><applet load="2fu5" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2fu5" size=" | + | |
caption="2fu5, resolution 2.00Å" /> | caption="2fu5, resolution 2.00Å" /> | ||
'''structure of Rab8 in complex with MSS4'''<br /> | '''structure of Rab8 in complex with MSS4'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Rab GTPases function as essential regulators of vesicle transport in | + | Rab GTPases function as essential regulators of vesicle transport in eukaryotic cells. MSS4 was shown to stimulate nucleotide exchange on Rab proteins associated with the exocytic pathway and to have nucleotide-free-Rab chaperone activity. A detailed kinetic analysis of MSS4 interaction with Rab8 showed that MSS4 is a relatively slow exchange factor that forms a long-lived nucleotide-free complex with RabGTPase. In contrast to other characterized exchange factor-GTPase complexes, MSS4:Rab8 complex binds GTP faster than GDP, but still ca. 3 orders of magnitude more slowly than comparable complexes. The crystal structure of the nucleotide-free MSS4:Rab8 complex revealed that MSS4 binds to the Switch I and interswitch regions of Rab8, forming an intermolecular beta-sheet. Complex formation results in dramatic structural changes of the Rab8 molecule, leading to unfolding of the nucleotide-binding site and surrounding structural elements, facilitating nucleotide release and slowing its rebinding. Coupling of nucleotide exchange activity to a cycle of GTPase unfolding and refolding represents a novel nucleotide exchange mechanism. |
==About this Structure== | ==About this Structure== | ||
| - | 2FU5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN and BME as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 2FU5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=BME:'>BME</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FU5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Mus musculus]] | [[Category: Mus musculus]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Goody, R | + | [[Category: Goody, R S.]] |
[[Category: Itzen, A.]] | [[Category: Itzen, A.]] | ||
[[Category: Pylypenko, O.]] | [[Category: Pylypenko, O.]] | ||
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[[Category: nucleotide exchange via unfolding of nucleotide binding region]] | [[Category: nucleotide exchange via unfolding of nucleotide binding region]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:25:06 2008'' |
Revision as of 15:25, 21 February 2008
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structure of Rab8 in complex with MSS4
Overview
Rab GTPases function as essential regulators of vesicle transport in eukaryotic cells. MSS4 was shown to stimulate nucleotide exchange on Rab proteins associated with the exocytic pathway and to have nucleotide-free-Rab chaperone activity. A detailed kinetic analysis of MSS4 interaction with Rab8 showed that MSS4 is a relatively slow exchange factor that forms a long-lived nucleotide-free complex with RabGTPase. In contrast to other characterized exchange factor-GTPase complexes, MSS4:Rab8 complex binds GTP faster than GDP, but still ca. 3 orders of magnitude more slowly than comparable complexes. The crystal structure of the nucleotide-free MSS4:Rab8 complex revealed that MSS4 binds to the Switch I and interswitch regions of Rab8, forming an intermolecular beta-sheet. Complex formation results in dramatic structural changes of the Rab8 molecule, leading to unfolding of the nucleotide-binding site and surrounding structural elements, facilitating nucleotide release and slowing its rebinding. Coupling of nucleotide exchange activity to a cycle of GTPase unfolding and refolding represents a novel nucleotide exchange mechanism.
About this Structure
2FU5 is a Protein complex structure of sequences from Homo sapiens and Mus musculus with and as ligands. Full crystallographic information is available from OCA.
Reference
Nucleotide exchange via local protein unfolding--structure of Rab8 in complex with MSS4., Itzen A, Pylypenko O, Goody RS, Alexandrov K, Rak A, EMBO J. 2006 Apr 5;25(7):1445-55. Epub 2006 Mar 16. PMID:16541104
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