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| - | [[Image:2cb6.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2cb6| PDB=2cb6 | SCENE= }} | | {{STRUCTURE_2cb6| PDB=2cb6 | SCENE= }} |
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| - | '''CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E195Q'''
| + | ===CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E195Q=== |
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| - | ==Overview==
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| - | The catalytic domain of a mosquitocidal toxin prolonged by a C-terminal 44 residue linker connecting to four ricin B-like domains was crystallized. Three crystal structures were established at resolutions between 2.5A and 3.0A using multi-wavelength and single-wavelength anomalous X-ray diffraction as well as molecular replacement phasing techniques. The chainfold of the toxin fragment corresponds to those of ADP-ribosylating enzymes. At pH 4.3 the fragment is associated in a C(7)-symmetric heptamer in agreement with an aggregate of similar size observed by size-exclusion chromatography. In two distinct crystal forms, the heptamers formed nearly spherical, D(7)-symmetric tetradecamers. Another crystal form obtained at pH 6.3 contained a recurring C(2)-symmetric tetramer, which, however, was not stable in solution. On the basis of the common chainfold and NAD(+)-binding site of all ADP-ribosyl transferases, the NAD(+)-binding site of the toxin was assigned at a high confidence level. In all three crystal forms the NAD(+) site was occupied by part of the 44 residue linker, explaining the known inhibitory effect of this polypeptide region. The structure showed that the cleavage site for toxin activation is in a highly mobile loop that is exposed in the monomer. Since it contains the inhibitory linker as a crucial part of the association contact, the observed heptamer is inactive. Moreover, the heptamer cannot be activated by proteolysis because the activation loop is at the ring center and not accessible for proteases. Therefore the heptamer, or possibly the tetradecamer, seems to represent an inactive storage form of the toxin.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16483607}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16483607 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16483607}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Adp-ribosyltransferase]] | | [[Category: Adp-ribosyltransferase]] |
| | [[Category: Toxin]] | | [[Category: Toxin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 21:38:08 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:13:41 2008'' |
Revision as of 06:13, 29 July 2008
Template:STRUCTURE 2cb6
CRYSTAL STRUCTURE OF THE CATALYTIC DOMAIN OF THE MOSQUITOCIDAL TOXIN FROM BACILLUS SPHAERICUS, MUTANT E195Q
Template:ABSTRACT PUBMED 16483607
About this Structure
2CB6 is a Single protein structure of sequence from Lysinibacillus sphaericus. Full crystallographic information is available from OCA.
Reference
Structure of the mosquitocidal toxin from Bacillus sphaericus., Reinert DJ, Carpusca I, Aktories K, Schulz GE, J Mol Biol. 2006 Apr 7;357(4):1226-36. Epub 2006 Jan 27. PMID:16483607
Page seeded by OCA on Tue Jul 29 09:13:41 2008
