1e1f
From Proteopedia
(New page: 200px<br /> <applet load="1e1f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1e1f, resolution 2.6Å" /> '''CRYSTAL STRUCTURE OF...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1E1F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Zea_mays Zea mays]] with PSG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E1F OCA]]. | + | 1E1F is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Zea_mays Zea mays]] with PSG as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Beta-glucosidase Beta-glucosidase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.21 3.2.1.21]]. Structure known Active Sites: CNA and CNB. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1E1F OCA]]. |
==Reference== | ==Reference== | ||
Crystal structure of a monocotyledon (maize ZMGlu1) beta-glucosidase and a model of its complex with p-nitrophenyl beta-D-thioglucoside., Czjzek M, Cicek M, Zamboni V, Burmeister WP, Bevan DR, Henrissat B, Esen A, Biochem J. 2001 Feb 15;354(Pt 1):37-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11171077 11171077] | Crystal structure of a monocotyledon (maize ZMGlu1) beta-glucosidase and a model of its complex with p-nitrophenyl beta-D-thioglucoside., Czjzek M, Cicek M, Zamboni V, Burmeister WP, Bevan DR, Henrissat B, Esen A, Biochem J. 2001 Feb 15;354(Pt 1):37-46. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11171077 11171077] | ||
| + | [[Category: Beta-glucosidase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Zea mays]] | [[Category: Zea mays]] | ||
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[[Category: retention of the anomeric configuration]] | [[Category: retention of the anomeric configuration]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:11:03 2007'' |
Revision as of 10:06, 30 October 2007
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CRYSTAL STRUCTURE OF A MONOCOT (MAIZE ZMGLU1) BETA-GLUCOSIDASE IN COMPLEX WITH P-NITROPHENYL-BETA-D-THIOGLUCOSIDE
Overview
The maize beta-glucosidase isoenzymes ZMGlu1 and ZMGlu2 hydrolyse the, abundant natural substrate DIMBOAGlc, (2-O-beta-D-glucopyranosyl-4-hydroxy-7-methoxy-1,4-benzoxazin-3-one), whose aglycone DIMBOA (2,4-hydroxy-7-methoxy-1,4-benzoxazin-3-one) is the, major defence chemical protecting seedlings and young plant parts against, herbivores and other pests. The two isoenzymes hydrolyse DIMBOAGlc with, similar kinetics but differ from each other and their sorghum homologues, with respect to specificity towards other substrates. To gain insights, into the mechanism of substrate (i.e. aglycone) specificity between the, two maize isoenzymes and their sorghum homologues, ZMGlu1 was produced in, Escherichia coli, purified, crystallized and its structure solved at 2.5, Angstrom resolution by X-ray ... [(full description)]
About this Structure
1E1F is a [Single protein] structure of sequence from [Zea mays] with PSG as [ligand]. Active as [Beta-glucosidase], with EC number [3.2.1.21]. Structure known Active Sites: CNA and CNB. Full crystallographic information is available from [OCA].
Reference
Crystal structure of a monocotyledon (maize ZMGlu1) beta-glucosidase and a model of its complex with p-nitrophenyl beta-D-thioglucoside., Czjzek M, Cicek M, Zamboni V, Burmeister WP, Bevan DR, Henrissat B, Esen A, Biochem J. 2001 Feb 15;354(Pt 1):37-46. PMID:11171077
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