2fxr
From Proteopedia
(New page: 200px<br /> <applet load="2fxr" size="450" color="white" frame="true" align="right" spinBox="true" caption="2fxr, resolution 2.50Å" /> '''human beta tryptase...) |
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| - | [[Image:2fxr.gif|left|200px]]<br /> | + | [[Image:2fxr.gif|left|200px]]<br /><applet load="2fxr" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2fxr" size=" | + | |
caption="2fxr, resolution 2.50Å" /> | caption="2fxr, resolution 2.50Å" /> | ||
'''human beta tryptase II complexed with activated ketone inhibitor CRA-29382'''<br /> | '''human beta tryptase II complexed with activated ketone inhibitor CRA-29382'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Improved peptide-based inhibitors of human beta tryptase were discovered | + | Improved peptide-based inhibitors of human beta tryptase were discovered using information gleaned from tripeptide library screening and structure-guided design methods, including fragment screening. Our efforts sought to improve this class of inhibitors by replacing the traditional Lys or Arg P1 element. The optimized compounds display low nanomolar potency against the mast cell target and several hundred-fold selectivity with respect to serine protease off targets. Thus, replacement of Lys/Arg at P1 in a peptide-like scaffold does not need to be accompanied by a loss in target affinity. |
==About this Structure== | ==About this Structure== | ||
| - | 2FXR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with C3A as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tryptase Tryptase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.59 3.4.21.59] Full crystallographic information is available from [http:// | + | 2FXR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=C3A:'>C3A</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Tryptase Tryptase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.59 3.4.21.59] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FXR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Tryptase]] | [[Category: Tryptase]] | ||
| - | [[Category: Katz, B | + | [[Category: Katz, B A.]] |
[[Category: C3A]] | [[Category: C3A]] | ||
[[Category: activated ketone inhibitor]] | [[Category: activated ketone inhibitor]] | ||
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[[Category: serine protease]] | [[Category: serine protease]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:26:10 2008'' |
Revision as of 15:26, 21 February 2008
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human beta tryptase II complexed with activated ketone inhibitor CRA-29382
Overview
Improved peptide-based inhibitors of human beta tryptase were discovered using information gleaned from tripeptide library screening and structure-guided design methods, including fragment screening. Our efforts sought to improve this class of inhibitors by replacing the traditional Lys or Arg P1 element. The optimized compounds display low nanomolar potency against the mast cell target and several hundred-fold selectivity with respect to serine protease off targets. Thus, replacement of Lys/Arg at P1 in a peptide-like scaffold does not need to be accompanied by a loss in target affinity.
About this Structure
2FXR is a Single protein structure of sequence from Homo sapiens with as ligand. Active as Tryptase, with EC number 3.4.21.59 Full crystallographic information is available from OCA.
Reference
Structure-guided design of peptide-based tryptase inhibitors., McGrath ME, Sprengeler PA, Hirschbein B, Somoza JR, Lehoux I, Janc JW, Gjerstad E, Graupe M, Estiarte A, Venkataramani C, Liu Y, Yee R, Ho JD, Green MJ, Lee CS, Liu L, Tai V, Spencer J, Sperandio D, Katz BA, Biochemistry. 2006 May 16;45(19):5964-73. PMID:16681368
Page seeded by OCA on Thu Feb 21 17:26:10 2008
