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2cm6

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{{STRUCTURE_2cm6| PDB=2cm6 | SCENE= }}
{{STRUCTURE_2cm6| PDB=2cm6 | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE C2B DOMAIN OF RABPHILIN3A'''
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===CRYSTAL STRUCTURE OF THE C2B DOMAIN OF RABPHILIN3A===
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==Overview==
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The Ca(2+) binding properties of C2 domains are essential for the function of their host proteins. We present here the first crystal structures showing an unexpected Ca(2+) binding mode of the C2B domain of rabphilin-3A in atomic detail. Acidic residues from the linker region between the C2A and C2B domains of rabphilin-3A interact with the Ca(2+)-binding region of the C2B domain. Because of these interactions, the coordination sphere of the two bound Ca(2+) ions is almost complete. Mutation of these acidic residues to alanine resulted in a 10-fold decrease in the intrinsic Ca(2+) binding affinity of the C2B domain. Using NMR spectroscopy, we show that this interaction occurred only in the Ca(2+)-bound state of the C2B domain. In addition, this Ca(2+) binding mode was maintained in the C2 domain tandem fragment. In NMR-based liposome binding assays, the linker was not released upon phospholipid binding. Therefore, this unprecedented Ca(2+) binding mode not only shows how a C2 domain increases its intrinsic Ca(2+) affinity, but also provides the structural base for an atypical protein-Ca(2+)-phospholipid binding mode of rabphilin-3A.
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{{ABSTRACT_PUBMED_17166855}}
==About this Structure==
==About this Structure==
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[[Category: Zinc]]
[[Category: Zinc]]
[[Category: Zinc-finger]]
[[Category: Zinc-finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 22:29:01 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:46:23 2008''

Revision as of 06:46, 29 July 2008

Image:2cm6.png

Template:STRUCTURE 2cm6

CRYSTAL STRUCTURE OF THE C2B DOMAIN OF RABPHILIN3A

Template:ABSTRACT PUBMED 17166855

About this Structure

2CM6 is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

The C2A-C2B linker defines the high affinity Ca(2+) binding mode of rabphilin-3A., Montaville P, Schlicker C, Leonov A, Zweckstetter M, Sheldrick GM, Becker S, J Biol Chem. 2007 Feb 16;282(7):5015-25. Epub 2006 Dec 13. PMID:17166855

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