2gbm
From Proteopedia
(New page: 200px<br /> <applet load="2gbm" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gbm, resolution 1.55Å" /> '''Crystal Structure o...) |
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| - | [[Image:2gbm.gif|left|200px]]<br /> | + | [[Image:2gbm.gif|left|200px]]<br /><applet load="2gbm" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="2gbm" size=" | + | |
caption="2gbm, resolution 1.55Å" /> | caption="2gbm, resolution 1.55Å" /> | ||
'''Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin'''<br /> | '''Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We previously concluded that, judging from NMR chemical shifts, the | + | We previously concluded that, judging from NMR chemical shifts, the effects of insertions into ubiquitin on its conformation appear to depend primarily on the site of insertion rather than the sequence of the insertion. To obtain a more complete and atomic-resolution understanding of how these insertions modulate the conformation of ubiquitin, we have solved the crystal structures of four insertional mutants of ubiquitin. Insertions between residues 9 and 10 of ubiquitin are minimally perturbing to the remainder of the protein, while larger alterations occur when the insertion is between residues 35 and 36. Further, the alterations in response to insertions are very similar for each mutant at a given site. Two insertions, one at each site, were designed from structurally homologous proteins. Interestingly, the secondary structure within these five to seven amino acid residue insertions is conserved in the new protein. Overall, the crystal structures support the previous conclusion that the conformational effects of these insertions are determined largely by the site of insertion and only secondarily by the sequence of the insert. |
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| + | ==Disease== | ||
| + | Known disease associated with this structure: Cleft palate, isolated OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191339 191339]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 2GBM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ARS as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 2GBM is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ARS:'>ARS</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GBM OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Ferraro, D | + | [[Category: Ferraro, D J.]] |
| - | [[Category: Ferraro, D | + | [[Category: Ferraro, D M.]] |
[[Category: Ramaswamy, S.]] | [[Category: Ramaswamy, S.]] | ||
| - | [[Category: Robertson, A | + | [[Category: Robertson, A D.]] |
[[Category: ARS]] | [[Category: ARS]] | ||
[[Category: loop insertion]] | [[Category: loop insertion]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:30:04 2008'' |
Revision as of 15:30, 21 February 2008
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Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin
Contents |
Overview
We previously concluded that, judging from NMR chemical shifts, the effects of insertions into ubiquitin on its conformation appear to depend primarily on the site of insertion rather than the sequence of the insertion. To obtain a more complete and atomic-resolution understanding of how these insertions modulate the conformation of ubiquitin, we have solved the crystal structures of four insertional mutants of ubiquitin. Insertions between residues 9 and 10 of ubiquitin are minimally perturbing to the remainder of the protein, while larger alterations occur when the insertion is between residues 35 and 36. Further, the alterations in response to insertions are very similar for each mutant at a given site. Two insertions, one at each site, were designed from structurally homologous proteins. Interestingly, the secondary structure within these five to seven amino acid residue insertions is conserved in the new protein. Overall, the crystal structures support the previous conclusion that the conformational effects of these insertions are determined largely by the site of insertion and only secondarily by the sequence of the insert.
Disease
Known disease associated with this structure: Cleft palate, isolated OMIM:[191339]
About this Structure
2GBM is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Structures of ubiquitin insertion mutants support site-specific reflex response to insertions hypothesis., Ferraro DM, Ferraro DJ, Ramaswamy S, Robertson AD, J Mol Biol. 2006 Jun 2;359(2):390-402. Epub 2006 Apr 5. PMID:16647719
Page seeded by OCA on Thu Feb 21 17:30:04 2008
