2cw1

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{{STRUCTURE_2cw1| PDB=2cw1 | SCENE= }}
{{STRUCTURE_2cw1| PDB=2cw1 | SCENE= }}
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'''Solution structure of the de novo-designed lambda Cro fold protein'''
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===Solution structure of the de novo-designed lambda Cro fold protein===
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==Overview==
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One of the classical DNA-binding proteins, bacteriophage lambda Cro, forms a homodimer with a unique fold of alpha-helices and beta-sheets. We have computationally designed an artificial sequence of 60 amino acid residues to stabilize the backbone tertiary structure of the lambda Cro dimer by simulated annealing using knowledge-based structure-sequence compatibility functions. The designed amino acid sequence has 25% identity with that of natural lambda Cro and preserves Phe58, which is important for formation of the stably folded structure of lambda Cro. The designed dimer protein and its monomeric variant, which was redesigned by the insertion of a beta-hairpin sequence at the C-terminal region to prevent dimerization, were synthesized and biochemically characterized to be well folded. The designed protein was monomeric under a wide range of protein concentrations and its solution structure was determined by NMR spectroscopy. The solved structure is similar to that of a monomeric variant of natural lambda Cro with a root-mean-square deviation of the polypeptide backbones at 2.1A and has a well-packed protein core. Thus, our knowledge-based functions provide approximate but essential relationships between amino acid sequences and protein structures, and are useful for finding novel sequences that are foldable into a given target structure.
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(as it appears on PubMed at http://www.pubmed.gov), where 16289118 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16289118}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW1 OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2CW1 OCA].
==Reference==
==Reference==
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[[Category: Shiro, Y.]]
[[Category: Shiro, Y.]]
[[Category: Lambda cro fold]]
[[Category: Lambda cro fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:11:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 09:45:33 2008''

Revision as of 06:45, 29 July 2008

Image:2cw1.png

Template:STRUCTURE 2cw1

Solution structure of the de novo-designed lambda Cro fold protein

Template:ABSTRACT PUBMED 16289118

About this Structure

Full experimental information is available from OCA.

Reference

Design of lambda Cro fold: solution structure of a monomeric variant of the de novo protein., Isogai Y, Ito Y, Ikeya T, Shiro Y, Ota M, J Mol Biol. 2005 Dec 9;354(4):801-14. Epub 2005 Oct 21. PMID:16289118

Page seeded by OCA on Tue Jul 29 09:45:33 2008

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