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2gl7

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(New page: 200px<br /> <applet load="2gl7" size="450" color="white" frame="true" align="right" spinBox="true" caption="2gl7, resolution 2.60&Aring;" /> '''Crystal Structure o...)
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'''Crystal Structure of a beta-catenin/BCL9/Tcf4 complex'''<br />
'''Crystal Structure of a beta-catenin/BCL9/Tcf4 complex'''<br />
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==About this Structure==
==About this Structure==
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2GL7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2GL7 OCA].
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2GL7 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GL7 OCA].
==Reference==
==Reference==
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[[Category: protein complex]]
[[Category: protein complex]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 12 22:19:53 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 17:28:28 2008''

Revision as of 15:28, 15 February 2008


2gl7, resolution 2.60Å

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Crystal Structure of a beta-catenin/BCL9/Tcf4 complex

Contents

Overview

The canonical Wnt pathway plays critical roles in embryonic development, stem cell growth, and tumorigenesis. Stimulation of the Wnt pathway leads, to the association of beta-catenin with Tcf and BCL9 in the nucleus, resulting in the transactivation of Wnt target genes. We have determined, the crystal structure of a beta-catenin/BCL9/Tcf-4 triple complex at 2.6 A, resolution. Our studies reveal that the beta-catenin binding site of BCL9, is distinct from that of most other beta-catenin partners and forms a good, target for developing drugs that block canonical Wnt/beta-catenin, signaling. The BCL9 beta-catenin binding domain (CBD) forms an alpha helix, that binds to the first armadillo repeat of beta-catenin, which can be, mutated to prevent beta-catenin binding to BCL9 without affecting cadherin, or alpha-catenin binding. We also demonstrate that beta-catenin Y142, phosphorylation, which has been proposed to regulate BCL9-2 binding, does, not directly affect the interaction of beta-catenin with either BCL9 or, BCL9-2.

Disease

Known diseases associated with this structure: Colorectal cancer OMIM:[116806], Diabetes mellitus, type 2, susceptibility to OMIM:[602228], Hepatoblastoma OMIM:[116806], Hepatocellular carcinoma OMIM:[116806], Ovarian carcinoma, endometrioid type OMIM:[116806], Pilomatricoma OMIM:[116806], Pitt-Hopkins syndrome OMIM:[602272]

About this Structure

2GL7 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of a beta-catenin/BCL9/Tcf4 complex., Sampietro J, Dahlberg CL, Cho US, Hinds TR, Kimelman D, Xu W, Mol Cell. 2006 Oct 20;24(2):293-300. PMID:17052462

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