2d1p

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{{STRUCTURE_2d1p| PDB=2d1p | SCENE= }}
{{STRUCTURE_2d1p| PDB=2d1p | SCENE= }}
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'''crystal structure of heterohexameric TusBCD proteins, which are crucial for the tRNA modification'''
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===crystal structure of heterohexameric TusBCD proteins, which are crucial for the tRNA modification===
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==Overview==
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Uridine at wobble position 34 of tRNA(Lys), tRNA(Glu), and tRNA(Gln) is exclusively modified into 2-thiouridine (s2U), which is crucial for both precise codon recognition and recognition by the cognate aminoacyl-tRNA synthetases. Recent Escherichia coli genetic studies revealed that the products of five novel genes, tusABCDE, function in the s2U modification. Here, we solved the 2.15 angstroms crystal structure of the E. coli TusBCD complex, a sulfur transfer mediator, forming a heterohexamer composed of a dimer of the heterotrimer. Structure-based sequence alignment suggested two putative active site Cys residues, Cys79 (in TusC) and Cys78 (in TusD), which are exposed on the hexameric complex. In vivo mutant analyses revealed that only Cys78, in the TusD subunit, participates in sulfur transfer during the s2U modification process. Since the single Cys acts as a catalytic residue, we proposed that TusBCD mediates sulfur relay via a putative persulfide state of the TusD subunit.
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The line below this paragraph, {{ABSTRACT_PUBMED_16472754}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16472754 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16472754}}
==About this Structure==
==About this Structure==
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[[Category: Sulfur transfer]]
[[Category: Sulfur transfer]]
[[Category: Trna modification]]
[[Category: Trna modification]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:31:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:49:34 2008''

Revision as of 10:49, 29 July 2008

Image:2d1p.png

Template:STRUCTURE 2d1p

crystal structure of heterohexameric TusBCD proteins, which are crucial for the tRNA modification

Template:ABSTRACT PUBMED 16472754

About this Structure

2D1P is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structural basis for sulfur relay to RNA mediated by heterohexameric TusBCD complex., Numata T, Fukai S, Ikeuchi Y, Suzuki T, Nureki O, Structure. 2006 Feb;14(2):357-66. PMID:16472754

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