2d7i

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{{STRUCTURE_2d7i| PDB=2d7i | SCENE= }}
{{STRUCTURE_2d7i| PDB=2d7i | SCENE= }}
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'''Crsytal structure of pp-GalNAc-T10 with UDP, GalNAc and Mn2+'''
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===Crsytal structure of pp-GalNAc-T10 with UDP, GalNAc and Mn2+===
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==Overview==
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Mucin-type O-glycans are important carbohydrate chains involved in differentiation and malignant transformation. Biosynthesis of the O-glycan is initiated by the transfer of N-acetylgalactosamine (GalNAc) which is catalyzed by UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases (pp-GalNAc-Ts). Here we present crystal structures of the pp-GalNAc-T10 isozyme, which has specificity for glycosylated peptides, in complex with the hydrolyzed donor substrate UDP-GalNAc and in complex with GalNAc-serine. A structural comparison with uncomplexed pp-GalNAc-T1 suggests that substantial conformational changes occur in two loops near the catalytic center upon donor substrate binding, and that a distinct interdomain arrangement between the catalytic and lectin domains forms a narrow cleft for acceptor substrates. The distance between the catalytic center and the carbohydrate-binding site on the lectin beta sub-domain influences the position of GalNAc glycosylation on GalNAc-glycosylated peptide substrates. A chimeric enzyme in which the two domains of pp-GalNAc-T10 are connected by a linker from pp-GalNAc-T1 acquires activity toward non-glycosylated acceptors, identifying a potential mechanism for generating the various acceptor specificities in different isozymes to produce a wide range of O-glycans.
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{{ABSTRACT_PUBMED_16650853}}
==About this Structure==
==About this Structure==
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[[Category: Beta trefoil]]
[[Category: Beta trefoil]]
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[[Category: Rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:16:49 2008''

Revision as of 17:16, 27 July 2008

Image:2d7i.png

Template:STRUCTURE 2d7i

Crsytal structure of pp-GalNAc-T10 with UDP, GalNAc and Mn2+

Template:ABSTRACT PUBMED 16650853

About this Structure

2D7I is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Structural basis of carbohydrate transfer activity by human UDP-GalNAc: polypeptide alpha-N-acetylgalactosaminyltransferase (pp-GalNAc-T10)., Kubota T, Shiba T, Sugioka S, Furukawa S, Sawaki H, Kato R, Wakatsuki S, Narimatsu H, J Mol Biol. 2006 Jun 9;359(3):708-27. Epub 2006 Apr 19. PMID:16650853

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