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2d97

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[[Image:2d97.gif|left|200px]]
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{{STRUCTURE_2d97| PDB=2d97 | SCENE= }}
{{STRUCTURE_2d97| PDB=2d97 | SCENE= }}
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'''Structure of VIL-xylanase'''
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===Structure of VIL-xylanase===
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==Overview==
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New techniques are presented for the preparation of iodine derivatives, involving vapour diffusion of iodine. Firstly, in the vaporizing iodine labelling (VIL) technique, a small amount of KI/I(2) solution is enclosed in a crystallization well, with the result that gaseous I(2) molecules diffuse into the crystallization droplets without exerting substantial changes in ionic strength in the target crystals. Once they have diffused into the droplet, the I(2) molecules sometimes iodinate accessible tyrosines at ortho positions. Secondly, when iodination is insufficient, the hydrogen peroxide VIL (HYPER-VIL) technique can be further applied to increase the iodination ratio by the addition of a small droplet of hydrogen peroxide (H(2)O(2)) to the crystallization well; the gaseous H(2)O(2) also diffuses into the crystallization droplet to emphasize the iodination. These techniques are most effective for phase determination when coupled with softer X-rays, such as those from Cu Kalpha or Cr Kalpha radiation. The effectiveness of these techniques was assessed using five different crystals. Four of the crystals were successfully iodinated, providing sufficient phasing power for structure determination.
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(as it appears on PubMed at http://www.pubmed.gov), where 16510975 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16510975}}
==About this Structure==
==About this Structure==
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[[Category: Yamano, A.]]
[[Category: Yamano, A.]]
[[Category: Iodo-tyrosine]]
[[Category: Iodo-tyrosine]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 23:55:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:01:11 2008''

Revision as of 03:01, 28 July 2008

Image:2d97.png

Template:STRUCTURE 2d97

Structure of VIL-xylanase

Template:ABSTRACT PUBMED 16510975

About this Structure

2D97 is a Single protein structure of sequence from Hypocrea jecorina. Full crystallographic information is available from OCA.

Reference

New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL)., Miyatake H, Hasegawa T, Yamano A, Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):280-9. Epub 2006, Feb 22. PMID:16510975

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