2dd5

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{{STRUCTURE_2dd5| PDB=2dd5 | SCENE= }}
{{STRUCTURE_2dd5| PDB=2dd5 | SCENE= }}
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'''Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus native holo-enzyme'''
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===Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus native holo-enzyme===
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==Overview==
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Thiocyanate hydrolase (SCNase) of Thiobacillus thioparus THI115 is a cobalt(III)-containing enzyme catalyzing the degradation of thiocyanate to carbonyl sulfide and ammonia. We determined the crystal structures of the apo- and native SCNases at a resolution of 2.0 A. SCNases in both forms had a conserved hetero-dodecameric structure, (alphabetagamma)(4). Four alphabetagamma hetero-trimers were structurally equivalent. One alphabetagamma hetero-trimer was composed of the core domain and the betaN domain, which was located at the center of the molecule and linked the hetero-trimers with novel quaternary interfaces. In both the apo- and native SCNases, the core domain was structurally conserved between those of iron and cobalt-types of nitrile hydratase (NHase). Native SCNase possessed the post-translationally modified cysteine ligands, gammaCys131-SO(2)H and gammaCys133-SOH like NHases. However, the low-spin cobalt(III) was found to be in the distorted square-pyramidal geometry, which had not been reported before in any protein. The size as well as the electrostatic properties of the substrate-binding pocket was totally different from NHases with respect to the charge distribution and the substrate accessibility, which rationally explains the differences in the substrate preference between SCNase and NHase.
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{{ABSTRACT_PUBMED_17222425}}
==About this Structure==
==About this Structure==
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[[Category: Sulfinic acid]]
[[Category: Sulfinic acid]]
[[Category: Thiocyanate]]
[[Category: Thiocyanate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:12:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:41:25 2008''

Revision as of 18:41, 27 July 2008

Image:2dd5.png

Template:STRUCTURE 2dd5

Thiocyanate hydrolase (SCNase) from Thiobacillus thioparus native holo-enzyme

Template:ABSTRACT PUBMED 17222425

About this Structure

2DD5 is a Protein complex structure of sequences from Thiobacillus thioparus. Full crystallographic information is available from OCA.

Reference

Structure of thiocyanate hydrolase: a new nitrile hydratase family protein with a novel five-coordinate cobalt(III) center., Arakawa T, Kawano Y, Kataoka S, Katayama Y, Kamiya N, Yohda M, Odaka M, J Mol Biol. 2007 Mar 9;366(5):1497-509. Epub 2006 Dec 8. PMID:17222425

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