2dep
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | [[Image:2dep. | + | {{Seed}} |
| + | [[Image:2dep.png|left|200px]] | ||
<!-- | <!-- | ||
| Line 9: | Line 10: | ||
{{STRUCTURE_2dep| PDB=2dep | SCENE= }} | {{STRUCTURE_2dep| PDB=2dep | SCENE= }} | ||
| - | + | ===Crystal Structure of xylanase B from Clostridium stercorarium F9=== | |
| - | |||
| - | ==Overview== | ||
| - | Recombinant mature xylanase B from Clostridium stercorarium has been prepared and crystallized by the sitting-drop vapour-diffusion method using 4 mg ml(-1) purified enzyme, 10.3%(w/v) polyethylene glycol 1500, 8.6%(v/v) glycerol and 0.34 M non-detergent sulfobetaine 195. A suitable crystal grew after incubation for ten weeks at 293 K. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 64.76, b = 96.60, c = 138.44 A. X-ray diffraction data were collected to 1.80 A resolution. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2DEP is a | + | 2DEP is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Clostridium_stercorarium Clostridium stercorarium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DEP OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:14747719</ref><ref group="xtra">PMID:15914908</ref><ref group="xtra">PMID:16233324</ref><references group="xtra"/> | |
[[Category: Clostridium stercorarium]] | [[Category: Clostridium stercorarium]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Fushinobu, S.]] | [[Category: Fushinobu, S.]] | ||
[[Category: Hayashi, K.]] | [[Category: Hayashi, K.]] | ||
| Line 33: | Line 30: | ||
[[Category: Structural genomic]] | [[Category: Structural genomic]] | ||
[[Category: Xylan degradation]] | [[Category: Xylan degradation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 04:49:41 2009'' | ||
Revision as of 02:49, 17 February 2009
Crystal Structure of xylanase B from Clostridium stercorarium F9
About this Structure
2DEP is a 2 chains structure of sequences from Clostridium stercorarium. Full crystallographic information is available from OCA.
Reference
- Nishimoto M, Fushinobu S, Miyanaga A, Wakagi T, Shoun H, Sakka K, Ohmiya K, Nirasawa S, Kitaoka M, Hayashi K. Crystallization and preliminary X-ray analysis of xylanase B from Clostridium stercorarium. Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):342-3. Epub 2004, Jan 23. PMID:14747719 doi:10.1107/S090744490302715X
- Nishimoto M, Kitaoka M, Fushinobu S, Hayashi K. The role of conserved arginine residue in loop 4 of glycoside hydrolase family 10 xylanases. Biosci Biotechnol Biochem. 2005 May;69(5):904-10. PMID:15914908
- Nishimoto M, Kitaoka M, Hayashi K. Employing chimeric xylanases to identify regions of an alkaline xylanase participating in enzyme activity at basic pH. J Biosci Bioeng. 2002;94(5):395-400. PMID:16233324
Page seeded by OCA on Tue Feb 17 04:49:41 2009
