2dfl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2dfl.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2dfl.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2dfl| PDB=2dfl | SCENE= }}
{{STRUCTURE_2dfl| PDB=2dfl | SCENE= }}
-
'''Crystal structure of left-handed RadA filament'''
+
===Crystal structure of left-handed RadA filament===
-
==Overview==
+
<!--
-
The RecA family of proteins mediates homologous recombination, an evolutionarily conserved pathway that maintains genomic stability by protecting against DNA double strand breaks. RecA proteins are thought to facilitate DNA strand exchange reactions as closed-rings or as right-handed helical filaments. Here, we report the crystal structure of a left-handed Sulfolobus solfataricus RadA helical filament. Each protomer in this left-handed filament is linked to its neighbour via interactions of a beta-strand polymerization motif with the neighbouring ATPase domain. Immediately following the polymerization motif, we identified an evolutionarily conserved hinge region (a subunit rotation motif) in which a 360 degrees clockwise axial rotation accompanies stepwise structural transitions from a closed ring to the AMP-PNP right-handed filament, then to an overwound right-handed filament and finally to the left-handed filament. Additional structural and functional analyses of wild-type and mutant proteins confirmed that the subunit rotation motif is crucial for enzymatic functions of RecA family proteins. These observations support the hypothesis that RecA family protein filaments may function as rotary motors.
+
The line below this paragraph, {{ABSTRACT_PUBMED_17329376}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 17329376 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_17329376}}
==About this Structure==
==About this Structure==
Line 35: Line 39:
[[Category: Reca]]
[[Category: Reca]]
[[Category: Recombination]]
[[Category: Recombination]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:19:45 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:32:32 2008''

Revision as of 05:32, 29 July 2008

Image:2dfl.png

Template:STRUCTURE 2dfl

Crystal structure of left-handed RadA filament

Template:ABSTRACT PUBMED 17329376

About this Structure

2DFL is a Single protein structure of sequence from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins., Chen LT, Ko TP, Chang YC, Lin KA, Chang CS, Wang AH, Wang TF, Nucleic Acids Res. 2007;35(6):1787-801. Epub 2007 Feb 28. PMID:17329376

Page seeded by OCA on Tue Jul 29 08:32:32 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2dfl"
Personal tools