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| - | [[Image:2dfs.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_2dfs| PDB=2dfs | SCENE= }} | | {{STRUCTURE_2dfs| PDB=2dfs | SCENE= }} |
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| - | '''3-D structure of Myosin-V inhibited state'''
| + | ===3-D structure of Myosin-V inhibited state=== |
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| - | ==Overview==
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| - | Unconventional myosin V (myoV) is an actin-based molecular motor that has a key function in organelle and mRNA transport, as well as in membrane trafficking. MyoV was the first member of the myosin superfamily shown to be processive, meaning that a single motor protein can 'walk' hand-over-hand along an actin filament for many steps before detaching. Full-length myoV has a low actin-activated MgATPase activity at low [Ca2+], whereas expressed constructs lacking the cargo-binding domain have a high activity regardless of [Ca2+] (refs 5-7). Hydrodynamic data and electron micrographs indicate that the active state is extended, whereas the inactive state is compact. Here we show the first three-dimensional structure of the myoV inactive state. Each myoV molecule consists of two heads that contain an amino-terminal motor domain followed by a lever arm that binds six calmodulins. The heads are followed by a coiled-coil dimerization domain (S2) and a carboxy-terminal globular cargo-binding domain. In the inactive structure, bending of myoV at the head-S2 junction places the cargo-binding domain near the motor domain's ATP-binding pocket, indicating that ATPase inhibition might occur through decreased rates of nucleotide exchange. The actin-binding interfaces are unobstructed, and the lever arm is oriented in a position typical of strong actin-binding states. This structure indicates that motor recycling after cargo delivery might occur through transport on actively treadmilling actin filaments rather than by diffusion.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_16625208}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16625208 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16625208}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Inhibited state]] | | [[Category: Inhibited state]] |
| | [[Category: Myosin-v]] | | [[Category: Myosin-v]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:19:58 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 10:08:38 2008'' |
Revision as of 07:08, 29 July 2008
Template:STRUCTURE 2dfs
3-D structure of Myosin-V inhibited state
Template:ABSTRACT PUBMED 16625208
About this Structure
2DFS is a Protein complex structure of sequences from Gallus gallus and Mus musculus. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of the myosin V inhibited state by cryoelectron tomography., Liu J, Taylor DW, Krementsova EB, Trybus KM, Taylor KA, Nature. 2006 Jul 13;442(7099):208-11. Epub 2006 Apr 16. PMID:16625208
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