2dko

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{{STRUCTURE_2dko| PDB=2dko | SCENE= }}
{{STRUCTURE_2dko| PDB=2dko | SCENE= }}
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'''Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis'''
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===Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis===
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==Overview==
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Caspases are cysteine proteases involved in the signalling cascades of programmed cell death in which caspase-3 plays a central role, since it propagates death signals from intrinsic and extrinsic stimuli to downstream targets. The atomic resolution (1.06 Angstroms) crystal structure of the caspase-3 DEVD-cmk complex reveals the structural basis for substrate selectivity in the S4 pocket. A low-barrier hydrogen bond is observed between the side-chains of the P4 inhibitor aspartic acid and Asp179 of the N-terminal tail of the symmetry related p12 subunit. Site-directed mutagenesis of Asp179 confirmed the significance of this residue in substrate recognition. In the 1.06 Angstroms crystal structure, a radiation damage induced rearrangement of the inhibitor methylketone moiety was observed. The carbon atom that in a substrate would represent the scissile peptide bond carbonyl carbon clearly shows a tetrahedral coordination and resembles the postulated tetrahedral intermediate of the acylation reaction.
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(as it appears on PubMed at http://www.pubmed.gov), where 16787777 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16787777}}
==About this Structure==
==About this Structure==
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[[Category: Radiation damage]]
[[Category: Radiation damage]]
[[Category: Tetrahedral intermediate]]
[[Category: Tetrahedral intermediate]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:37:26 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 05:44:21 2008''

Revision as of 02:44, 29 July 2008

Image:2dko.png

Template:STRUCTURE 2dko

Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis

Template:ABSTRACT PUBMED 16787777

About this Structure

2DKO is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Extended substrate recognition in caspase-3 revealed by high resolution X-ray structure analysis., Ganesan R, Mittl PR, Jelakovic S, Grutter MG, J Mol Biol. 2006 Jun 23;359(5):1378-88. Epub 2006 May 11. PMID:16787777

Page seeded by OCA on Tue Jul 29 05:44:21 2008

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