2dkv
From Proteopedia
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{{STRUCTURE_2dkv| PDB=2dkv | SCENE= }} | {{STRUCTURE_2dkv| PDB=2dkv | SCENE= }} | ||
| - | + | ===Crystal structure of class I chitinase from Oryza sativa L. japonica=== | |
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| - | ==Overview== | ||
| - | Rice chitinases are encoded by a small multigene family. To clarify the overall organization of rice chitinase genes, we have isolated and characterized the genes Cht-1, Cht-2 and Cht-3. Although all the three genes encode class I chitinase, the nucleotide sequences of the coding regions of Cht-1 and Cht-3 are very similar (90%), while that of Cht-2 is clearly more divergent (78%). Only Cht-2 has a 130 bp intron and encodes a C-terminal peptide sequence similar to that known to function as a vacuolar targeting signal. In 5' flanking regions of Cht-1 and Cht-3, but not of Cht-2, conserved sequences (GGCCGGCYGCCCYAG) were found. Related sequences were found also in the 5' flanking regions of another chitinase gene and a beta-glucanase gene which has also been reported to be stress-induced in rice. RNA blot hybridization analysis demonstrated that the stress-induced expression patterns of the Cht-1 and Cht-3 genes are similar, but quite different from that of Cht-2. However, all three genes are active in unstressed roots. By restriction fragment length polymorphism (RFLP) linkage analysis, Cht-1 and Cht-3 were mapped onto chromosome 6 and shown to be closely linked (0.8 cM). Cht-2 was mapped onto chromosome 5. All these features suggest that the expression patterns of rice class I chitinase genes may be correlated with their levels of sequence divergence and their chromosomal location. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2DKV is a | + | 2DKV is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Oryza_sativa Oryza sativa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DKV OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:7901749</ref><ref group="xtra">PMID:15327374</ref><references group="xtra"/> | |
[[Category: Chitinase]] | [[Category: Chitinase]] | ||
[[Category: Oryza sativa]] | [[Category: Oryza sativa]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Kezuka, Y.]] | [[Category: Kezuka, Y.]] | ||
[[Category: Nishizawa, Y.]] | [[Category: Nishizawa, Y.]] | ||
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[[Category: Oryza sativa l. japonica]] | [[Category: Oryza sativa l. japonica]] | ||
[[Category: Whole structure]] | [[Category: Whole structure]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 10:37:03 2009'' | ||
Revision as of 08:37, 17 February 2009
Crystal structure of class I chitinase from Oryza sativa L. japonica
About this Structure
2DKV is a 1 chain structure of sequence from Oryza sativa. Full crystallographic information is available from OCA.
Reference
- Nishizawa Y, Kishimoto N, Saito A, Hibi T. Sequence variation, differential expression and chromosomal location of rice chitinase genes. Mol Gen Genet. 1993 Oct;241(1-2):1-10. PMID:7901749
- Kezuka Y, Kitazaki K, Itoh Y, Watanabe J, Takaha O, Watanabe T, Nishizawa Y, Nonaka T. Crystallization and preliminary X-ray analysis of plant class I chitinase from rice. Protein Pept Lett. 2004 Aug;11(4):401-5. PMID:15327374
Page seeded by OCA on Tue Feb 17 10:37:03 2009
