2dog

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{{STRUCTURE_2dog| PDB=2dog | SCENE= }}
{{STRUCTURE_2dog| PDB=2dog | SCENE= }}
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'''Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8'''
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===Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8===
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==Overview==
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The RimM protein has been implicated in the maturation of the 30S ribosomal subunit. It binds to ribosomal protein S19, located in the head domain of the 30S subunit. Multiple sequence alignments predicted that RimM possesses two domains in its N- and C-terminal regions. In the present study, we have produced Thermus thermophilus RimM in both the full-length form (162 residues) and its N-terminal fragment, spanning residues 1 to 85, as soluble proteins in Escherichia coli and have performed structural analyses by nuclear magnetic resonance spectroscopy. Residues 1 to 80 of the RimM protein fold into a single structural domain adopting a six-stranded beta-barrel fold. On the other hand, the C-terminal region of RimM (residues 81 to 162) is partly folded in solution. Analyses of 1H-15N heteronuclear single quantum correlation spectra revealed that a wide range of residues in the C-terminal region, as well as the residues in the vicinity of a hydrophobic patch in the N-terminal domain, were dramatically affected upon complex formation with ribosomal protein S19.
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{{ABSTRACT_PUBMED_17616598}}
==About this Structure==
==About this Structure==
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2DOG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DOG OCA].
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2DOG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DOG OCA].
==Reference==
==Reference==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Unknown function]]
[[Category: Unknown function]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 00:52:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:02:45 2008''

Revision as of 10:02, 28 July 2008

Image:2dog.png

Template:STRUCTURE 2dog

Solution structure of the N-terminal domain of RimM from Thermus thermophilus HB8

Template:ABSTRACT PUBMED 17616598

About this Structure

2DOG is a Single protein structure of sequence from Thermus thermophilus. Full experimental information is available from OCA.

Reference

Structural characterization of the ribosome maturation protein, RimM., Suzuki S, Tatsuguchi A, Matsumoto E, Kawazoe M, Kaminishi T, Shirouzu M, Muto Y, Takemoto C, Yokoyama S, J Bacteriol. 2007 Sep;189(17):6397-406. Epub 2007 Jul 6. PMID:17616598

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