2dt9
From Proteopedia
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{{STRUCTURE_2dt9| PDB=2dt9 | SCENE= }} | {{STRUCTURE_2dt9| PDB=2dt9 | SCENE= }} | ||
| - | + | ===Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus=== | |
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| - | ==Overview== | ||
| - | To reveal the catalytic mechanism of Thermus aspartate kinase, each of 29 amino acid residues that were highly conserved in the sequenced aspartate kinases, was replaced with alanine or leucine by PCR site-directed mutagenesis. Comparison of the kinetic parameters of these mutants with those of the wild-type aspartate kinase suggested that Thr47 was involved in binding aspartate and that Lys7 and Glu74 were involved in catalysis. Analysis of the effective concentrations of magnesium ion on the activity showed that the mutants with replacements at Ser41, Thr47, Asp154 and Asp182 required higher concentrations of magnesium ion. This suggests that these four residues play important roles in the binding of magnesium ions which are required for enzymatic activity. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2DT9 is a | + | 2DT9 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DT9 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16232547</ref><ref group="xtra">PMID:7773416</ref><references group="xtra"/> | |
[[Category: Aspartate kinase]] | [[Category: Aspartate kinase]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Thermus thermophilus]] | [[Category: Thermus thermophilus]] | ||
[[Category: Fushinobu, S.]] | [[Category: Fushinobu, S.]] | ||
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[[Category: Protein-ligand complex]] | [[Category: Protein-ligand complex]] | ||
[[Category: Regulatory subunit]] | [[Category: Regulatory subunit]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:46:47 2009'' | ||
Revision as of 22:46, 17 February 2009
Crystal structure of the regulatory subunit of aspartate kinase from Thermus flavus
About this Structure
2DT9 is a 2 chains structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
- Kobashi N, Nishiyama M, Tanokura M. Kinetic and mutation analyses of aspartate kinase from Thermus flavus. J Biosci Bioeng. 1999;87(6):739-45. PMID:16232547
- Nishiyama M, Kukimoto M, Beppu T, Horinouchi S. An operon encoding aspartokinase and purine phosphoribosyltransferase in Thermus flavus. Microbiology. 1995 May;141 ( Pt 5):1211-9. PMID:7773416
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