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2dxn

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{{STRUCTURE_2dxn| PDB=2dxn | SCENE= }}
{{STRUCTURE_2dxn| PDB=2dxn | SCENE= }}
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'''Glycerophosphodiesterase from Enterobacter aerogenes'''
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===Glycerophosphodiesterase from Enterobacter aerogenes===
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==Overview==
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The structure of the glycerophosphodiesterase (GDPD) from Enterobacter aerogenes, GpdQ, has been solved by SAD phasing from the active site metal ions. Structural analysis indicates that GpdQ belongs to the alpha/beta sandwich metallo-phosphoesterase family, rather than the (alpha/beta)(8) barrel GDPD family, suggesting that GpdQ is a structurally novel GDPD. Hexameric GpdQ is generated by interactions between three dimers. The dimers are formed through domain swapping, stabilised by an inter-chain disulfide bond, and beta-sheet extension. The active site contains a binuclear metal centre, with a fully occupied alpha-metal ion site, and partially occupied beta-metal ion site, as revealed by anomalous scattering analysis. Using a combination of TLS refinement and normal mode analysis, the dynamic movement of GpdQ was investigated. This analysis suggests that the hexameric quaternary structure stabilises the base of the dimer, which promotes "breathing" of the active site cleft. Comparison with other metallo-phosphodiesterases shows that although the central, catalytic, domain is highly conserved, many of these enzymes possess structurally unrelated secondary domains located at the entrance of the active site. We suggest that this could be a common structural feature of metallo-phosphodiesterases that constrains substrate specificity, preventing non-specific phosphodiester hydrolysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 17306828 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17306828}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The structure and function of a novel glycerophosphodiesterase from Enterobacter aerogenes., Jackson CJ, Carr PD, Liu JW, Watt SJ, Beck JL, Ollis DL, J Mol Biol. 2007 Apr 6;367(4):1047-62. Epub 2007 Jan 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17306828 17306828]
The structure and function of a novel glycerophosphodiesterase from Enterobacter aerogenes., Jackson CJ, Carr PD, Liu JW, Watt SJ, Beck JL, Ollis DL, J Mol Biol. 2007 Apr 6;367(4):1047-62. Epub 2007 Jan 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17306828 17306828]
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The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes., Jackson CJ, Carr PD, Kim HK, Liu JW, Ollis DL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):659-61. Epub 2006 Jun 10. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16820687 16820687]
[[Category: Enterobacter aerogenes]]
[[Category: Enterobacter aerogenes]]
[[Category: Glycerophosphodiester phosphodiesterase]]
[[Category: Glycerophosphodiester phosphodiesterase]]
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[[Category: Domain-swapping]]
[[Category: Domain-swapping]]
[[Category: Metalloenzyme]]
[[Category: Metalloenzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 01:34:31 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:22:38 2008''

Revision as of 16:22, 28 July 2008

Image:2dxn.png

Template:STRUCTURE 2dxn

Glycerophosphodiesterase from Enterobacter aerogenes

Template:ABSTRACT PUBMED 17306828

About this Structure

2DXN is a Single protein structure of sequence from Enterobacter aerogenes. Full crystallographic information is available from OCA.

Reference

The structure and function of a novel glycerophosphodiesterase from Enterobacter aerogenes., Jackson CJ, Carr PD, Liu JW, Watt SJ, Beck JL, Ollis DL, J Mol Biol. 2007 Apr 6;367(4):1047-62. Epub 2007 Jan 20. PMID:17306828

The purification, crystallization and preliminary diffraction of a glycerophosphodiesterase from Enterobacter aerogenes., Jackson CJ, Carr PD, Kim HK, Liu JW, Ollis DL, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jul 1;62(Pt, 7):659-61. Epub 2006 Jun 10. PMID:16820687

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