2hiu
From Proteopedia
(New page: 200px<br /> <applet load="2hiu" size="450" color="white" frame="true" align="right" spinBox="true" caption="2hiu" /> '''NMR STRUCTURE OF HUMAN INSULIN IN 20% ACETI...) |
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| - | [[Image:2hiu.gif|left|200px]]<br /> | + | [[Image:2hiu.gif|left|200px]]<br /><applet load="2hiu" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2hiu" /> | caption="2hiu" /> | ||
'''NMR STRUCTURE OF HUMAN INSULIN IN 20% ACETIC ACID, ZINC-FREE, 10 STRUCTURES'''<br /> | '''NMR STRUCTURE OF HUMAN INSULIN IN 20% ACETIC ACID, ZINC-FREE, 10 STRUCTURES'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We have determined the structure of a metastable disulphide isomer of | + | We have determined the structure of a metastable disulphide isomer of human insulin. Although not observed for proinsulin folding or insulin-chain recombination, the isomer retains ordered secondary structure and a compact hydrophobic core. Comparison with native insulin reveals a global rearrangement in the orientation of A- and B-chains. One face of the protein's surface is nevertheless in common between native and non-native structures. This face contains receptor-binding determinants, rationalizing the partial biological activity of the isomer. Structures of native and non-native disulphide isomers also define alternative three-dimensional templates. Threading of insulin-like sequences provide an experimental realization of the inverse protein-folding problem. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2HIU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure | + | 2HIU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry 1HIU. The following page contains interesting information on the relation of 2HIU with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb14_1.html Insulin]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2HIU OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Insulin]] | [[Category: Insulin]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Chance, R | + | [[Category: Chance, R E.]] |
| - | [[Category: Frank, B | + | [[Category: Frank, B H.]] |
| - | [[Category: Gozani, S | + | [[Category: Gozani, S N.]] |
| - | [[Category: Hoffmann, J | + | [[Category: Hoffmann, J A.]] |
| - | [[Category: Hua, Q | + | [[Category: Hua, Q X.]] |
| - | [[Category: Weiss, M | + | [[Category: Weiss, M A.]] |
[[Category: glucose metabolism]] | [[Category: glucose metabolism]] | ||
[[Category: hormone]] | [[Category: hormone]] | ||
[[Category: insulin]] | [[Category: insulin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:42:21 2008'' |
Revision as of 15:42, 21 February 2008
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NMR STRUCTURE OF HUMAN INSULIN IN 20% ACETIC ACID, ZINC-FREE, 10 STRUCTURES
Contents |
Overview
We have determined the structure of a metastable disulphide isomer of human insulin. Although not observed for proinsulin folding or insulin-chain recombination, the isomer retains ordered secondary structure and a compact hydrophobic core. Comparison with native insulin reveals a global rearrangement in the orientation of A- and B-chains. One face of the protein's surface is nevertheless in common between native and non-native structures. This face contains receptor-binding determinants, rationalizing the partial biological activity of the isomer. Structures of native and non-native disulphide isomers also define alternative three-dimensional templates. Threading of insulin-like sequences provide an experimental realization of the inverse protein-folding problem.
Disease
Known diseases associated with this structure: Diabetes mellitus, rare form OMIM:[176730], Hyperproinsulinemia, familial OMIM:[176730], MODY, one form OMIM:[176730]
About this Structure
2HIU is a Protein complex structure of sequences from Homo sapiens. This structure supersedes the now removed PDB entry 1HIU. The following page contains interesting information on the relation of 2HIU with [Insulin]. Full crystallographic information is available from OCA.
Reference
Structure of a protein in a kinetic trap., Hua QX, Gozani SN, Chance RE, Hoffmann JA, Frank BH, Weiss MA, Nat Struct Biol. 1995 Feb;2(2):129-38. PMID:7749917
Page seeded by OCA on Thu Feb 21 17:42:21 2008
