2e7p

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{{STRUCTURE_2e7p| PDB=2e7p | SCENE= }}
{{STRUCTURE_2e7p| PDB=2e7p | SCENE= }}
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'''Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides'''
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===Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides===
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==Overview==
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When expressed in Escherichia coli, cytosolic poplar glutaredoxin C1 (CGYC active site) exists as a dimeric iron-sulfur-containing holoprotein or as a monomeric apoprotein in solution. Analytical and spectroscopic studies of wild-type protein and site-directed variants and structural characterization of the holoprotein by using x-ray crystallography indicate that the holoprotein contains a subunit-bridging [2Fe-2S] cluster that is ligated by the catalytic cysteines of two glutaredoxins and the cysteines of two glutathiones. Mutagenesis data on a variety of poplar glutaredoxins suggest that the incorporation of an iron-sulfur cluster could be a general feature of plant glutaredoxins possessing a glycine adjacent to the catalytic cysteine. In light of these results, the possible involvement of plant glutaredoxins in oxidative stress sensing or iron-sulfur biosynthesis is discussed with respect to their intracellular localization.
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(as it appears on PubMed at http://www.pubmed.gov), where 17460036 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17460036}}
==About this Structure==
==About this Structure==
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[[Category: Poplar]]
[[Category: Poplar]]
[[Category: Thioredoxin fold]]
[[Category: Thioredoxin fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:05:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:04:05 2008''

Revision as of 00:04, 28 July 2008

Template:STRUCTURE 2e7p

Crystal structure of the holo form of glutaredoxin C1 from populus tremula x tremuloides

Template:ABSTRACT PUBMED 17460036

About this Structure

2E7P is a Single protein structure of sequence from Populus tremula x populus tremuloides. Full crystallographic information is available from OCA.

Reference

Functional, structural, and spectroscopic characterization of a glutathione-ligated [2Fe-2S] cluster in poplar glutaredoxin C1., Rouhier N, Unno H, Bandyopadhyay S, Masip L, Kim SK, Hirasawa M, Gualberto JM, Lattard V, Kusunoki M, Knaff DB, Georgiou G, Hase T, Johnson MK, Jacquot JP, Proc Natl Acad Sci U S A. 2007 May 1;104(18):7379-84. Epub 2007 Apr 25. PMID:17460036

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