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2e9r

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[[Image:2e9r.gif|left|200px]]
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{{STRUCTURE_2e9r| PDB=2e9r | SCENE= }}
{{STRUCTURE_2e9r| PDB=2e9r | SCENE= }}
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'''Foot-and-mouth disease virus RNA-dependent RNA polymerase in complex with a template-primer RNA and with ribavirin'''
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===Foot-and-mouth disease virus RNA-dependent RNA polymerase in complex with a template-primer RNA and with ribavirin===
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==Overview==
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RNA virus replication is an error-prone event caused by the low fidelity of viral RNA-dependent RNA polymerases. Replication fidelity can be decreased further by the use of mutagenic ribonucleoside analogs to a point where viral genetic information can no longer be maintained. For foot-and-mouth disease virus, the antiviral analogs ribavirin and 5-fluorouracil have been shown to be mutagenic, contributing to virus extinction through lethal mutagenesis. Here, we report the x-ray structure of four elongation complexes of foot-and-mouth disease virus polymerase 3D obtained in presence of natural substrates, ATP and UTP, or mutagenic nucleotides, ribavirin triphosphate and 5-fluorouridine triphosphate with different RNAs as template-primer molecules. The ability of these complexes to synthesize RNA in crystals allowed us to capture different successive replication events and to define the critical amino acids involved in (i) the recognition and positioning of the incoming nucleotide or analog; (ii) the positioning of the acceptor base of the template strand; and (iii) the positioning of the 3'-OH group of the primer nucleotide during RNA replication. The structures identify key interactions involved in viral RNA replication and provide insights into the molecular basis of the low fidelity of viral RNA polymerases.
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The line below this paragraph, {{ABSTRACT_PUBMED_17517631}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 17517631 is the PubMed ID number.
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{{ABSTRACT_PUBMED_17517631}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Sequential structures provide insights into the fidelity of RNA replication., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9463-8. Epub 2007 May 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17517631 17517631]
Sequential structures provide insights into the fidelity of RNA replication., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9463-8. Epub 2007 May 21. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17517631 17517631]
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The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16456546 16456546]
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A comparison of viral RNA-dependent RNA polymerases., Ferrer-Orta C, Arias A, Escarmis C, Verdaguer N, Curr Opin Struct Biol. 2006 Feb;16(1):27-34. Epub 2005 Dec 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16364629 16364629]
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Structure of foot-and-mouth disease virus RNA-dependent RNA polymerase and its complex with a template-primer RNA., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, J Biol Chem. 2004 Nov 5;279(45):47212-21. Epub 2004 Aug 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15294895 15294895]
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Mutant viral polymerase in the transition of virus to error catastrophe identifies a critical site for RNA binding., Arias A, Agudo R, Ferrer-Orta C, Perez-Luque R, Airaksinen A, Brocchi E, Domingo E, Verdaguer N, Escarmis C, J Mol Biol. 2005 Nov 11;353(5):1021-32. Epub 2005 Sep 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16216271 16216271]
[[Category: Foot-and-mouth disease virus]]
[[Category: Foot-and-mouth disease virus]]
[[Category: RNA-directed RNA polymerase]]
[[Category: RNA-directed RNA polymerase]]
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[[Category: Polymerase]]
[[Category: Polymerase]]
[[Category: Rna-dependent rna polymerase]]
[[Category: Rna-dependent rna polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 02:12:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 14:17:50 2008''

Revision as of 11:17, 28 July 2008

Image:2e9r.png

Template:STRUCTURE 2e9r

Foot-and-mouth disease virus RNA-dependent RNA polymerase in complex with a template-primer RNA and with ribavirin

Template:ABSTRACT PUBMED 17517631

About this Structure

2E9R is a Single protein structure of sequence from Foot-and-mouth disease virus. Full crystallographic information is available from OCA.

Reference

Sequential structures provide insights into the fidelity of RNA replication., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, Proc Natl Acad Sci U S A. 2007 May 29;104(22):9463-8. Epub 2007 May 21. PMID:17517631

The structure of a protein primer-polymerase complex in the initiation of genome replication., Ferrer-Orta C, Arias A, Agudo R, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, EMBO J. 2006 Feb 22;25(4):880-8. Epub 2006 Feb 2. PMID:16456546

A comparison of viral RNA-dependent RNA polymerases., Ferrer-Orta C, Arias A, Escarmis C, Verdaguer N, Curr Opin Struct Biol. 2006 Feb;16(1):27-34. Epub 2005 Dec 20. PMID:16364629

Structure of foot-and-mouth disease virus RNA-dependent RNA polymerase and its complex with a template-primer RNA., Ferrer-Orta C, Arias A, Perez-Luque R, Escarmis C, Domingo E, Verdaguer N, J Biol Chem. 2004 Nov 5;279(45):47212-21. Epub 2004 Aug 3. PMID:15294895

Mutant viral polymerase in the transition of virus to error catastrophe identifies a critical site for RNA binding., Arias A, Agudo R, Ferrer-Orta C, Perez-Luque R, Airaksinen A, Brocchi E, Domingo E, Verdaguer N, Escarmis C, J Mol Biol. 2005 Nov 11;353(5):1021-32. Epub 2005 Sep 26. PMID:16216271

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