2i5d
From Proteopedia
(New page: 200px<br /> <applet load="2i5d" size="450" color="white" frame="true" align="right" spinBox="true" caption="2i5d, resolution 1.630Å" /> '''Crystal Structure ...) |
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| - | [[Image:2i5d.gif|left|200px]]<br /> | + | [[Image:2i5d.gif|left|200px]]<br /><applet load="2i5d" size="350" color="white" frame="true" align="right" spinBox="true" |
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caption="2i5d, resolution 1.630Å" /> | caption="2i5d, resolution 1.630Å" /> | ||
'''Crystal Structure of Human Inosine Triphosphate Pyrophosphatase'''<br /> | '''Crystal Structure of Human Inosine Triphosphate Pyrophosphatase'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of human inosine triphosphate pyrophosphohydrolase (ITPA) | + | The structure of human inosine triphosphate pyrophosphohydrolase (ITPA) has been determined using diffraction data to 1.6 A resolution. ITPA contributes to the accurate replication of DNA by cleansing cellular dNTP pools of mutagenic nucleotide purine analogs such as dITP or dXTP. A similar high-resolution unpublished structure has been deposited in the Protein Data Bank from a monoclinic and pseudo-merohedrally twinned crystal. Here, cocrystallization of ITPA with a molar ratio of XTP appears to have improved the crystals by eliminating twinning and resulted in an orthorhombic space group. However, there was no evidence for bound XTP in the structure. Comparison with substrate-bound NTPase from a thermophilic organism predicts the movement of residues within helix alpha1, the loop before alpha6 and helix alpha7 to cap off the active site when substrate is bound. |
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 2I5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Nucleoside-triphosphate_diphosphatase Nucleoside-triphosphate diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.19 3.6.1.19] Full crystallographic information is available from [http:// | + | 2I5D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Active as [http://en.wikipedia.org/wiki/Nucleoside-triphosphate_diphosphatase Nucleoside-triphosphate diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.19 3.6.1.19] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I5D OCA]. |
==Reference== | ==Reference== | ||
| - | Structure of the orthorhombic form of human inosine triphosphate pyrophosphatase., Porta J, Kolar C, Kozmin SG, Pavlov YI, Borgstahl GE, Acta | + | Structure of the orthorhombic form of human inosine triphosphate pyrophosphatase., Porta J, Kolar C, Kozmin SG, Pavlov YI, Borgstahl GE, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1076-81. Epub 2006 Oct 25. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17077483 17077483] |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Nucleoside-triphosphate diphosphatase]] | [[Category: Nucleoside-triphosphate diphosphatase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Borgstahl, G | + | [[Category: Borgstahl, G E.O.]] |
| - | [[Category: Kozmin, S | + | [[Category: Kozmin, S G.]] |
| - | [[Category: Pavlov, Y | + | [[Category: Pavlov, Y I.]] |
| - | [[Category: Porta, J | + | [[Category: Porta, J C.]] |
[[Category: monomeric protein]] | [[Category: monomeric protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 17:49:18 2008'' |
Revision as of 15:49, 21 February 2008
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Crystal Structure of Human Inosine Triphosphate Pyrophosphatase
Contents |
Overview
The structure of human inosine triphosphate pyrophosphohydrolase (ITPA) has been determined using diffraction data to 1.6 A resolution. ITPA contributes to the accurate replication of DNA by cleansing cellular dNTP pools of mutagenic nucleotide purine analogs such as dITP or dXTP. A similar high-resolution unpublished structure has been deposited in the Protein Data Bank from a monoclinic and pseudo-merohedrally twinned crystal. Here, cocrystallization of ITPA with a molar ratio of XTP appears to have improved the crystals by eliminating twinning and resulted in an orthorhombic space group. However, there was no evidence for bound XTP in the structure. Comparison with substrate-bound NTPase from a thermophilic organism predicts the movement of residues within helix alpha1, the loop before alpha6 and helix alpha7 to cap off the active site when substrate is bound.
Disease
Known disease associated with this structure: Inosine triphosphatase deficiency OMIM:[147520]
About this Structure
2I5D is a Single protein structure of sequence from Homo sapiens. Active as Nucleoside-triphosphate diphosphatase, with EC number 3.6.1.19 Full crystallographic information is available from OCA.
Reference
Structure of the orthorhombic form of human inosine triphosphate pyrophosphatase., Porta J, Kolar C, Kozmin SG, Pavlov YI, Borgstahl GE, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1076-81. Epub 2006 Oct 25. PMID:17077483
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