This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


2er8

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:2er8.gif|left|200px]]
+
{{Seed}}
 +
[[Image:2er8.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_2er8| PDB=2er8 | SCENE= }}
{{STRUCTURE_2er8| PDB=2er8 | SCENE= }}
-
'''Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex'''
+
===Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex===
-
==Overview==
+
<!--
-
Gal4 is the prototypical Zn2Cys6 binuclear cluster transcriptional regulator that binds as a homodimer to DNA containing inverted CGG half-sites. Leu3, a member of this protein family, binds to everted (opposite polarity to inverted) CGG half-sites, and an H50C mutation within the Leu3 Zn2Cys6 binuclear motif abolishes its transcriptional repression function without impairing DNA binding. We report the X-ray crystal structures of DNA complexes with Leu3 and Leu3(H50C) and solution DNA binding studies of selected Leu3 mutant proteins. These studies reveal the molecular details of everted CGG half-site recognition, and suggest a role for the H50C mutation in transcriptional repression. Comparison with the Gal4-DNA complex shows an unexpected conservation in the DNA recognition mode of inverted and everted CGG half-sites, and points to a critical function of a linker region between the Zn2Cys6 binuclear cluster and dimerization regions in DNA binding specificity. Broader implications of these findings are discussed.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16615914}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16615914 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16615914}}
==About this Structure==
==About this Structure==
Line 24: Line 28:
[[Category: Fitzgerald, M X.]]
[[Category: Fitzgerald, M X.]]
[[Category: Marmorstein, R.]]
[[Category: Marmorstein, R.]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:01:40 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 21:27:23 2008''

Revision as of 18:27, 27 July 2008

Image:2er8.png

Template:STRUCTURE 2er8

Crystal Structure of Leu3 DNA-binding domain complexed with a 12mer DNA duplex

Template:ABSTRACT PUBMED 16615914

About this Structure

2ER8 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.

Reference

Structure of a Leu3-DNA complex: recognition of everted CGG half-sites by a Zn2Cys6 binuclear cluster protein., Fitzgerald MX, Rojas JR, Kim JM, Kohlhaw GB, Marmorstein R, Structure. 2006 Apr;14(4):725-35. PMID:16615914

Page seeded by OCA on Sun Jul 27 21:27:23 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2er8"
Personal tools