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2esd

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{{STRUCTURE_2esd| PDB=2esd | SCENE= }}
{{STRUCTURE_2esd| PDB=2esd | SCENE= }}
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'''Crystal Structure of thioacylenzyme intermediate of an Nadp Dependent Aldehyde Dehydrogenase'''
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===Crystal Structure of thioacylenzyme intermediate of an Nadp Dependent Aldehyde Dehydrogenase===
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==Overview==
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Crystal structures of several members of the nonphosphorylating CoA-independent aldehyde dehydrogenase (ALDH) family have shown that the peculiar binding mode of the cofactor to the Rossmann fold results in a conformational flexibility for the nicotinamide moiety of the cofactor. This has been hypothesized to constitute an essential feature of the catalytic mechanism because the conformation of the cofactor required for the acylation step is not appropriate for the deacylation step. In the present study, the structure of a reaction intermediate of the E268A-glyceraldehyde 3-phosphate dehydrogenase (GAPN) from Streptococcus mutans, obtained by soaking the crystals of the enzyme/NADP complex with the natural substrate, is reported. The substrate is bound covalently in the four monomers and presents the geometric characteristics expected for a thioacylenzyme intermediate. Control experiments assessed that reduction of the coenzyme has occurred within the crystal. The structure reveals that reduction of the cofactor upon acylation leads to an extensive motion of the nicotinamide moiety with a flip of the reduced pyridinium ring away from the active site without significant changes of the protein structure. This event positions the reduced nicotinamide moiety in a pocket that likely constitutes the exit door for NADPH. Arguments are provided that the structure reported here constitutes a reasonable picture of the first thioacylenzyme intermediate characterized thus far in the ALDH family and that the position of the reduced nicotinamide moiety observed in GAPN is the one suitable for the deacylation step within all of the nonphosphorylating CoA-independent ALDH family.
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{{ABSTRACT_PUBMED_16503652}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
The first crystal structure of a thioacylenzyme intermediate in the ALDH family: new coenzyme conformation and relevance to catalysis., D'Ambrosio K, Pailot A, Talfournier F, Didierjean C, Benedetti E, Aubry A, Branlant G, Corbier C, Biochemistry. 2006 Mar 7;45(9):2978-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16503652 16503652]
The first crystal structure of a thioacylenzyme intermediate in the ALDH family: new coenzyme conformation and relevance to catalysis., D'Ambrosio K, Pailot A, Talfournier F, Didierjean C, Benedetti E, Aubry A, Branlant G, Corbier C, Biochemistry. 2006 Mar 7;45(9):2978-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16503652 16503652]
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Structural and biochemical investigations of the catalytic mechanism of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans., Cobessi D, Tete-Favier F, Marchal S, Branlant G, Aubry A, J Mol Biol. 2000 Jun 30;300(1):141-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10864505 10864505]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Streptococcus mutans]]
[[Category: Streptococcus mutans]]
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[[Category: Gapn]]
[[Category: Gapn]]
[[Category: Ternary complex]]
[[Category: Ternary complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:03:48 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:07:22 2008''

Revision as of 10:07, 28 July 2008

Image:2esd.png

Template:STRUCTURE 2esd

Crystal Structure of thioacylenzyme intermediate of an Nadp Dependent Aldehyde Dehydrogenase

Template:ABSTRACT PUBMED 16503652

About this Structure

2ESD is a Single protein structure of sequence from Streptococcus mutans. Full crystallographic information is available from OCA.

Reference

The first crystal structure of a thioacylenzyme intermediate in the ALDH family: new coenzyme conformation and relevance to catalysis., D'Ambrosio K, Pailot A, Talfournier F, Didierjean C, Benedetti E, Aubry A, Branlant G, Corbier C, Biochemistry. 2006 Mar 7;45(9):2978-86. PMID:16503652

Structural and biochemical investigations of the catalytic mechanism of an NADP-dependent aldehyde dehydrogenase from Streptococcus mutans., Cobessi D, Tete-Favier F, Marchal S, Branlant G, Aubry A, J Mol Biol. 2000 Jun 30;300(1):141-52. PMID:10864505

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