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2ez9

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{{STRUCTURE_2ez9| PDB=2ez9 | SCENE= }}
{{STRUCTURE_2ez9| PDB=2ez9 | SCENE= }}
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'''Pyruvate oxidase variant F479W in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate'''
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===Pyruvate oxidase variant F479W in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate===
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==Overview==
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Enzymes that use the cofactor thiamin diphosphate (ThDP, 1), the biologically active form of vitamin B(1), are involved in numerous metabolic pathways in all organisms. Although a theory of the cofactor's underlying reaction mechanism has been established over the last five decades, the three-dimensional structures of most major reaction intermediates of ThDP enzymes have remained elusive. Here, we report the X-ray structures of key intermediates in the oxidative decarboxylation of pyruvate, a central reaction in carbon metabolism catalyzed by the ThDP- and flavin-dependent enzyme pyruvate oxidase (POX)3 from Lactobacillus plantarum. The structures of 2-lactyl-ThDP (LThDP, 2) and its stable phosphonate analog, of 2-hydroxyethyl-ThDP (HEThDP, 3) enamine and of 2-acetyl-ThDP (AcThDP, 4; all shown bound to the enzyme's active site) provide profound insights into the chemical mechanisms and the stereochemical course of thiamin catalysis. These snapshots also suggest a mechanism for a phosphate-linked acyl transfer coupled to electron transfer in a radical reaction of pyruvate oxidase.
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(as it appears on PubMed at http://www.pubmed.gov), where 16680160 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16680160}}
==About this Structure==
==About this Structure==
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[[Category: Reaction intermediate analogue]]
[[Category: Reaction intermediate analogue]]
[[Category: Tpp enzyme]]
[[Category: Tpp enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 03:16:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:34:25 2008''

Revision as of 16:34, 28 July 2008

Image:2ez9.png

Template:STRUCTURE 2ez9

Pyruvate oxidase variant F479W in complex with reaction intermediate analogue 2-phosphonolactyl-thiamin diphosphate

Template:ABSTRACT PUBMED 16680160

About this Structure

2EZ9 is a Single protein structure of sequence from Lactobacillus plantarum. Full crystallographic information is available from OCA.

Reference

The catalytic cycle of a thiamin diphosphate enzyme examined by cryocrystallography., Wille G, Meyer D, Steinmetz A, Hinze E, Golbik R, Tittmann K, Nat Chem Biol. 2006 Jun;2(6):324-8. Epub 2006 May 7. PMID:16680160

Page seeded by OCA on Mon Jul 28 19:34:25 2008

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